丝状真菌抗真菌药敏试验标准化程序的多中心评估
Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi.
作者信息
Espinel-Ingroff A, Bartlett M, Bowden R, Chin N X, Cooper C, Fothergill A, McGinnis M R, Menezes P, Messer S A, Nelson P W, Odds F C, Pasarell L, Peter J, Pfaller M A, Rex J H, Rinaldi M G, Shankland G S, Walsh T J, Weitzman I
机构信息
Medical College of Virginia/Virginia Commonwealth University, Richmond 23298-0049, USA.
出版信息
J Clin Microbiol. 1997 Jan;35(1):139-43. doi: 10.1128/jcm.35.1.139-143.1997.
Abstract
A multicenter study was conducted to expand the generation and analysis of data that supports the proposal of a reference method for the antifungal susceptibility testing of filamentous fungi. Broth microdilution MICs of amphotericin B and itraconazole were determined in 11 centers against 30 coded duplicate pairs of Aspergillus spp., Fusarium spp., Pseudallescheria boydii, and Rhizopus arrhizus. The effect of inoculum density (approximately 10(3) and 10(4) CFU/ml), incubation time (24, 48, and 72 h), and procedure of MIC determination (conventional and colorimetric [Alamar Blue] evaluation of growth inhibition) on intra- and interlaboratory agreement was analyzed. Based on intra- (97 to 100%) and interlaboratory (94 to 95%) agreement for both drugs, the overall optimal testing conditions identified were determination of colorimetric MICs after 48 to 72 h of incubation with an inoculum density of approximately 10(4) CFU/ml. These testing conditions are proposed as guidelines for a reference broth microdilution method.
摘要
开展了一项多中心研究，以扩大数据的生成和分析，这些数据支持丝状真菌抗真菌药敏试验参考方法的提议。在11个中心针对30对编码重复的曲霉属、镰刀菌属、波氏假阿利什霉和米根霉测定了两性霉素B和伊曲康唑的肉汤微量稀释MIC。分析了接种物密度（约10³和10⁴CFU/ml）、孵育时间（24、48和72小时）以及MIC测定程序（生长抑制的传统和比色法[阿拉玛蓝]评估）对实验室内和实验室间一致性的影响。基于两种药物的实验室内（97%至100%）和实验室间（94%至95%）一致性，确定的总体最佳测试条件为接种物密度约10⁴CFU/ml、孵育48至72小时后测定比色法MIC。这些测试条件被提议作为参考肉汤微量稀释法的指南。
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