从克隆的互补脱氧核糖核酸拯救牛瘟病毒。
Rescue of rinderpest virus from cloned cDNA.
作者信息
Baron M D, Barrett T
机构信息
Institute for Animal Health, Pirbright, Surrey, United Kingdom.
出版信息
J Virol. 1997 Feb;71(2):1265-71. doi: 10.1128/JVI.71.2.1265-1271.1997.
Abstract
Rinderpest virus is a morbillivirus and is the causative agent of a widespread and important disease of cattle. The viral genome is a single strand of RNA in the negative sense. We have constructed plasmids containing cDNA copies of the 5' and 3' termini of the virus separated by a reporter gene and have shown that antigenome-sense RNA transcripts of these model genomes can be replicated, transcribed, and packaged by helper virus, both rinderpest virus and the related measles virus. Further, these genome analogs can be replicated and transcribed by viral proteins expressed from cDNA clones by using a recombinant vaccinia virus expressing T7 RNA polymerase (MVA-T7). Using this latter system, we have rescued live rinderpest virus from a full-length cDNA copy of the genome of the RBOK vaccine strain. The recombinant virus appears to grow in tissue culture identically to the original virus.
摘要
牛瘟病毒是一种麻疹病毒，是牛的一种广泛且重要疾病的病原体。病毒基因组是一条负链单链RNA。我们构建了含有病毒5'和3'末端cDNA拷贝的质粒，中间由一个报告基因隔开，并表明这些模型基因组的反基因组正义RNA转录本可以被辅助病毒（牛瘟病毒和相关的麻疹病毒）复制、转录和包装。此外，通过使用表达T7 RNA聚合酶的重组痘苗病毒（MVA-T7），这些基因组类似物可以被cDNA克隆表达的病毒蛋白复制和转录。利用后一种系统，我们从RBOK疫苗株基因组的全长cDNA拷贝中拯救出了活的牛瘟病毒。重组病毒在组织培养中的生长情况似乎与原始病毒完全相同。
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