评估重组Em18和亲和纯化Em18在肺泡型棘球蚴病与囊型棘球蚴病及其他寄生虫感染的血清学鉴别诊断中的应用。
Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections.
作者信息
Xiao Ning, Mamuti Wulamu, Yamasaki Hiroshi, Sako Yasuhito, Nakao Minoru, Nakaya Kazuhiro, Gottstein Bruno, Schantz Peter M, Lightowlers Marshall W, Craig Philip S, Ito Akira
机构信息
Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan and Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
出版信息
J Clin Microbiol. 2003 Jul;41(7):3351-3. doi: 10.1128/JCM.41.7.3351-3353.2003.
Abstract
To further evaluate recombinant Em18 antigen (rEm18) for immunodiagnosis of human alveolar echinococcosis, 208 serum samples were examined by enzyme-linked immunosorbent assay (ELISA). To comparatively assess the results of rEm18-ELISA, ELISA and immunoblot analysis with two affinity-purified native antigens were also performed with 45 selected serum samples. The results indicate that rEm18 is highly useful for serodiagnosis.
摘要
为进一步评估重组Em18抗原（rEm18）用于人体肺泡型棘球蚴病的免疫诊断，采用酶联免疫吸附测定（ELISA）检测了208份血清样本。为比较评估rEm18-ELISA的结果，还对45份选定的血清样本进行了ELISA以及用两种亲和纯化的天然抗原进行免疫印迹分析。结果表明，rEm18对血清学诊断非常有用。
相似文献
1
Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections.
J Clin Microbiol. 2003 Jul;41(7):3351-3. doi: 10.1128/JCM.41.7.3351-3353.2003.
2
Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test.
J Clin Microbiol. 2002 Nov;40(11):4161-5. doi: 10.1128/JCM.40.11.4161-4165.2002.
3
Alveolar echinococcosis: characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18.
J Clin Microbiol. 2002 Aug;40(8):2760-5. doi: 10.1128/JCM.40.8.2760-2765.2002.
4
Development of Em18-immunoblot and Em18-ELISA for specific diagnosis of alveolar echinococcosis.
Acta Trop. 2003 Feb;85(2):173-82. doi: 10.1016/s0001-706x(02)00221-8.
5
Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France.
Parasite. 2014;21:34. doi: 10.1051/parasite/2014037. Epub 2014 Jul 25.
6
Use of the ELISA (Em2-Em18) and Western Blotting Methods on Diagnosis of Alveolar Echinococcosis.
Turkiye Parazitol Derg. 2019 Oct 7;43(Suppl 1):13-17. doi: 10.4274/tpd.galenos.2019.6447.
7
Differential serodiagnosis of cystic and alveolar echinococcosis using native and recombinant antigens in Japan.
Southeast Asian J Trop Med Public Health. 2001;32 Suppl 2:111-5.
8
Immunodiagnosis of polycystic hydatid disease/polycystic echinococcosis due to Echinococcus vogeli.
Am J Trop Med Hyg. 1995 Nov;53(5):558-63. doi: 10.4269/ajtmh.1995.53.558.
9
Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis.
Parasite Immunol. 2000 Nov;22(11):553-9. doi: 10.1046/j.1365-3024.2000.00336.x.
10
Recombinant AgB8/1 ELISA test vs. commercially available IgG ELISA test in the diagnosis of cystic echinococcosis.
Parasite Immunol. 2013 Dec;35(12):433-40. doi: 10.1111/pim.12050.
引用本文的文献
1
Advances in Novel Diagnostic Techniques for Alveolar Echinococcosis.
Diagnostics (Basel). 2025 Feb 27;15(5):585. doi: 10.3390/diagnostics15050585.
2
Primary Retroperitoneal Hydatid Cyst: A Diagnostic and Treatment Conundrum.
Cureus. 2024 Feb 8;16(2):e53842. doi: 10.7759/cureus.53842. eCollection 2024 Feb.
3
Progress of research on the diagnosis and treatment of bone cystic echinococcosis.
Front Microbiol. 2023 Oct 18;14:1273870. doi: 10.3389/fmicb.2023.1273870. eCollection 2023.
4
Serological Screening of Patients Diagnosed with Alveolar Echinococcus Disease in Their Home Regions.
Iran J Parasitol. 2022 Oct-Dec;17(4):554-561. doi: 10.18502/ijpa.v17i4.11283.
5
Diagnostic and follow-up performance of serological tests for different forms/courses of alveolar echinococcosis.
Food Waterborne Parasitol. 2019 May 8;16:e00055. doi: 10.1016/j.fawpar.2019.e00055. eCollection 2019 Sep.
6
Dogs as victims of their own worms: Serodiagnosis of canine alveolar echinococcosis.
Parasit Vectors. 2017 Sep 16;10(1):422. doi: 10.1186/s13071-017-2369-0.
7
Serological validation of an alveolar echinococcosis rat model with a single hepatic lesion.
J Vet Med Sci. 2017 Feb 14;79(2):308-313. doi: 10.1292/jvms.16-0513. Epub 2016 Nov 27.
8
Cystic pulmonary hydatidosis.
Lung India. 2016 Mar-Apr;33(2):179-91. doi: 10.4103/0970-2113.177449.
9
Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France.
Parasite. 2014;21:34. doi: 10.1051/parasite/2014037. Epub 2014 Jul 25.
10
Surgical resection of hepatic cystic echinococcosis impaired by preoperative diagnosis.
Case Rep Med. 2013;2013:271256. doi: 10.1155/2013/271256. Epub 2013 Dec 18.
本文引用的文献
1
Immunodiagnostic and molecular approaches for the detection of taeniid cestode infections.
Trends Parasitol. 2003 Sep;19(9):377-81. doi: 10.1016/s1471-4922(03)00200-9.
2
Control of echinococcosis and cysticercosis: a public health challenge to international cooperation in China.
Acta Trop. 2003 Apr;86(1):3-17. doi: 10.1016/s0001-706x(02)00269-3.
3
Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test.
J Clin Microbiol. 2002 Nov;40(11):4161-5. doi: 10.1128/JCM.40.11.4161-4165.2002.
4
Alveolar echinococcosis: characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18.
J Clin Microbiol. 2002 Aug;40(8):2760-5. doi: 10.1128/JCM.40.8.2760-2765.2002.
5
Usefulness of hydatid cyst fluid of Echinococcus granulosus developed in mice with secondary infection for serodiagnosis of cystic Echinococcosis in humans.
Clin Diagn Lab Immunol. 2002 May;9(3):573-6. doi: 10.1128/cdli.9.3.573-576.2002.
6
An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China.
Acta Trop. 2000 Nov 2;77(2):167-77. doi: 10.1016/s0001-706x(00)00134-0.
7
Immunodiagnosis of alveolar echinococcosis by enzyme-linked immunosorbent assay using a partially purified Em18/16 enriched fraction.
Clin Diagn Lab Immunol. 1997 Jan;4(1):57-9. doi: 10.1128/cdli.4.1.57-59.1997.
8
Echinococcus multilocularis: molecular and immunochemical characterization of diagnostic antigen II/3-10.
Parasitology. 1993 Sep;107 ( Pt 3):335-42. doi: 10.1017/s0031182000079300.
9
Comparative analysis of full-length antigen II/3 from Echinococcus multilocularis and E. granulosus.
Parasitology. 1994 Aug;109 ( Pt 2):223-32. doi: 10.1017/s0031182000076344.
Zotero 插件