Xiao Ning, Mamuti Wulamu, Yamasaki Hiroshi, Sako Yasuhito, Nakao Minoru, Nakaya Kazuhiro, Gottstein Bruno, Schantz Peter M, Lightowlers Marshall W, Craig Philip S, Ito Akira
Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan and Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
J Clin Microbiol. 2003 Jul;41(7):3351-3. doi: 10.1128/JCM.41.7.3351-3353.2003.
To further evaluate recombinant Em18 antigen (rEm18) for immunodiagnosis of human alveolar echinococcosis, 208 serum samples were examined by enzyme-linked immunosorbent assay (ELISA). To comparatively assess the results of rEm18-ELISA, ELISA and immunoblot analysis with two affinity-purified native antigens were also performed with 45 selected serum samples. The results indicate that rEm18 is highly useful for serodiagnosis.
为进一步评估重组Em18抗原（rEm18）用于人体肺泡型棘球蚴病的免疫诊断，采用酶联免疫吸附测定（ELISA）检测了208份血清样本。为比较评估rEm18-ELISA的结果，还对45份选定的血清样本进行了ELISA以及用两种亲和纯化的天然抗原进行免疫印迹分析。结果表明，rEm18对血清学诊断非常有用。
