Dikalov S, Grigor'ev I A, Voinov M, Bassenge E
Institute of Chemical Kinetics & Combustion, Novosibirsk, Russia.
Biochem Biophys Res Commun. 1998 Jul 20;248(2):211-5. doi: 10.1006/bbrc.1998.8936.
The reactions of the new sterically hindered hydroxylamine 1-hydroxy-4-phosphonooxy-2,2,6,6-tetramethylpiperidine (PP-H) with superoxide radical and peroxynitrite have been studied. These reactions produce the nitroxide 4-phosphonooxy-2,2,6, 6-tetramethyl-piperidinyloxy. The rate constant for reaction of superoxide with PP-H is determined as (8.4+/-0.6).10(2) M-1s-1. It was found that PP-H provides almost the same spin trapping efficacy as 1-hydroxy-3-carboxy-pyrrolidine (CP-H). The background oxidation of PP-H in blood is much less than for CP-H. The extremely slow PP-H penetration into the cells makes possible the study of extracellular formation of superoxide radical. The acute treatment of blood with nitroglycerin is shown to induce an extracellular superoxide radical formation. PP-H is more sensitive for detection of reactive oxygen species as compared with CP-H. PP-H is an effective scavenger of superoxide radical and of peroxynitrite, and can be used to quantify the extracellular formation of these reactive oxygen species.
新型空间位阻羟胺1-羟基-4-膦酰氧基-2,2,6,6-四甲基哌啶（PP-H）与超氧自由基和过氧亚硝酸根的反应已得到研究。这些反应生成氮氧化物4-膦酰氧基-2,2,6,6-四甲基哌啶氧基。超氧与PP-H反应的速率常数确定为(8.4±0.6)·10² M⁻¹s⁻¹。发现PP-H提供的自旋捕获效率与1-羟基-3-羧基吡咯烷（CP-H）几乎相同。PP-H在血液中的背景氧化远低于CP-H。PP-H极缓慢地渗透进入细胞使得研究细胞外超氧自由基的形成成为可能。用硝酸甘油对血液进行急性处理显示可诱导细胞外超氧自由基的形成。与CP-H相比，PP-H对活性氧的检测更敏感。PP-H是超氧自由基和过氧亚硝酸根的有效清除剂，可用于定量这些活性氧在细胞外的形成。
