Ibuki K, Ido E, Setiyaningsih S, Yamashita M, Agus L R, Takehisa J, Miura T, Dondin S, Hayami M
Laboratory of Pathogenic Virus, Institute for Virus Research, Kyoto University, Sakyo-ku.
Jpn J Cancer Res. 1997 Jan;88(1):1-4. doi: 10.1111/j.1349-7006.1997.tb00293.x.
To study the evolutionary origin of human T-lymphotropic virus type I/simian T-lymphotropic virus type I (HTLV-I/STLV-I), we isolated and characterized STLV-I from orangutans (Pongo pygmaeus). Plasma samples from 3 out of 41 animals examined were reactive by particle agglutination and immunofluorescence, and one of these three was confirmed to be anti-HTLV-I antibody-positive by western blotting (WB). Cultured peripheral blood mononuclear cells from the WB-positive orangutan were reactive to anti-STLV-I-positive rhesus monkey plasma. The proviral long terminal repeat region was amplified by polymerase chain reaction and sequenced. A phylogenetic analysis indicated that orangutan STLV-I is related to the Melanesian group of HTLV-Is and other Asian STLV-Is, but the degree of divergence is considerable.
为研究人类嗜T淋巴细胞病毒I型/猿猴嗜T淋巴细胞病毒I型（HTLV-I/STLV-I）的进化起源，我们从猩猩（婆罗洲猩猩）中分离并鉴定了STLV-I。在检测的41只动物中，有3只的血浆样本通过颗粒凝集和免疫荧光呈阳性反应，其中1只经蛋白质印迹法（WB）确认为抗HTLV-I抗体阳性。来自WB阳性猩猩的培养外周血单个核细胞与抗STLV-I阳性恒河猴血浆发生反应。通过聚合酶链反应扩增前病毒长末端重复区域并进行测序。系统发育分析表明，猩猩STLV-I与HTLV-I的美拉尼西亚组及其他亚洲STLV-I相关，但差异程度相当大。
