Yamashita M, Achiron A, Miura T, Takehisa J, Ido E, Igarashi T, Ibuki K, Osame M, Sonoda S, Melamed E
Laboratory of Pathogenic Virus, Kyoto University, Japan.
Virus Genes. 1995;10(1):85-90. doi: 10.1007/BF01724300.
A new endemic focus of human T-lymphotropic virus type I (HTLV-I) was recently reported among Mashhadi Jews, a group of immigrants from northeastern Iran to Israel. We extracted DNAs from fresh peripheral blood mononuclear cells (PBMCs) and/or gargle mouthwash from 10 HTLV-I carriers, who consisted of members of one family, and HTLV-I-associated myelopathy (HAM) and adult T-cell leukemia (ATL) patients. Long terminal repeat (LTR) regions of proviral DNAs were sequenced and analyzed phylogenetically. In a phylogenetic tree, all the Mashhadi HTLV-I isolates belonged to subtype A, one of the three subtypes of the cosmopolitan type of HTLV-I, and made a tight cluster distinct from the other isolates of subtype A from Japan, India, the Caribbean Basin, and South America. Although a few nucleotide substitutions were observed among the clones sequenced, no characteristic sequence variation was found in different disease manifestations, even in one family or different sources of DNA preparation.
最近有报道称，在从伊朗东北部移民到以色列的马什哈德犹太人中出现了一个新的人类嗜T淋巴细胞病毒I型（HTLV-I）地方性疫源地。我们从10名HTLV-I携带者的新鲜外周血单核细胞（PBMC）和/或漱口液中提取了DNA，这些携带者包括一个家族的成员以及HTLV-I相关脊髓病（HAM）和成人T细胞白血病（ATL）患者。对前病毒DNA的长末端重复序列（LTR）区域进行了测序，并进行了系统发育分析。在系统发育树中，所有马什哈德HTLV-I分离株均属于A亚型，这是全球流行型HTLV-I的三种亚型之一，并且形成了一个紧密的聚类，与来自日本、印度、加勒比海盆地和南美洲的其他A亚型分离株不同。尽管在测序的克隆中观察到了一些核苷酸替换，但在不同的疾病表现中，即使在一个家族或不同的DNA制备来源中，也未发现特征性的序列变异。
