Meisinger C, Grothe C
Institute of Anatomy, University of Freiburg, Germany.
J Neurochem. 1997 Mar;68(3):1150-8. doi: 10.1046/j.1471-4159.1997.68031150.x.
To study the functional role of endogenous basic fibroblast growth factor-2 (FGF-2) during degeneration and regeneration of the sensory system, we have determined the expression and regulation of FGF-2 and FGF receptor (FGFR)-1 mRNAs in spinal ganglia and sciatic nerve during experimental transection and crush injury of the sciatic nerve. In contrast to levels of the FGFR-1 transcript, which is not altered, the level of FGF-2 mRNA is dramatically up-regulated in spinal ganglia after injury. In the proximal and distal nerve stumps both transcript levels are significantly elevated, albeit at different time points. The FGF-2 isoforms are differently up-regulated in spinal ganglia and sciatic nerve following peripheral nerve lesion. The differential response of FGF-2 mRNA and protein and of FGFR-1 mRNA in spinal ganglia and sciatic nerve after lesion is suggestive of different physiological functions: a local reaction at the lesion site where axonal regrowth occurs and a trophic reaction for the degenerating/regenerating sensory neurons.
为研究内源性碱性成纤维细胞生长因子-2（FGF-2）在感觉系统退变和再生过程中的功能作用，我们测定了坐骨神经实验性横断和挤压损伤过程中，脊髓神经节和坐骨神经中FGF-2和FGF受体（FGFR）-1 mRNA的表达及调控情况。与未改变的FGFR-1转录本水平不同，损伤后脊髓神经节中FGF-2 mRNA水平显著上调。在近端和远端神经残端，尽管时间点不同，但两种转录本水平均显著升高。周围神经损伤后，脊髓神经节和坐骨神经中FGF-2亚型的上调情况不同。损伤后脊髓神经节和坐骨神经中FGF-2 mRNA、蛋白及FGFR-1 mRNA的差异反应提示其具有不同的生理功能：在轴突再生发生的损伤部位的局部反应，以及对退变/再生感觉神经元的营养反应。
