一种从血浆中提取1型人类免疫缺陷病毒RNA的快速简便方法：提高了灵敏度。
A rapid and simple method for extracting human immunodeficiency virus type 1 RNA from plasma: enhanced sensitivity.
作者信息
Mulder J, Resnick R, Saget B, Scheibel S, Herman S, Payne H, Harrigan R, Kwok S
机构信息
Roche Molecular Systems, Alameda, San Francisco, California, USA.
出版信息
J Clin Microbiol. 1997 May;35(5):1278-80. doi: 10.1128/jcm.35.5.1278-1280.1997.
Abstract
We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations and requires approximately 1.5 h for the processing of 24 samples. Viral recovery ranges from 80 to 90%, with an analytical sensitivity approaching 20 copies/ml.
摘要
我们在此介绍一种通过高速离心处理人类免疫缺陷病毒（HIV）感染血浆的快速简便技术。从多达1毫升血浆中沉淀出1型HIV病毒颗粒，用非离子去污剂轻轻裂解，然后直接进行扩增。该程序操作步骤少，处理24个样本大约需要1.5小时。病毒回收率在80%至90%之间，分析灵敏度接近20拷贝/毫升。
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