通过NucliSens核酸序列扩增法和Quantiplex分支DNA分析法检测血浆中1型人类免疫缺陷病毒RNA水平的比较。
Comparison of levels of human immunodeficiency virus type 1 RNA in plasma as measured by the NucliSens nucleic acid sequence-based amplification and Quantiplex branched-DNA assays.
作者信息
Ginocchio C C, Tetali S, Washburn D, Zhang F, Kaplan M H
机构信息
Departments of Medicine and Clinical Laboratories, North Shore University Hospital-NYU School of Medicine, Manhasset, New York, USA.
出版信息
J Clin Microbiol. 1999 Apr;37(4):1210-2. doi: 10.1128/JCM.37.4.1210-1212.1999.
Abstract
This study compared levels of human immunodeficiency virus type 1 RNA in plasma as measured by the Quantiplex branched-DNA and NucliSens nucleic acid sequence-based amplification assays. RNA was detectable in 118 of 184 samples (64.13%) by the Quantiplex assay and in 171 of 184 samples (92.94%) by the NucliSens assay. Regression analysis indicated that a linear relationship existed between the two sets of values (P < 0.0001), although the Quantiplex and NucliSens values were significantly different (P < 0.001), with the NucliSens values being approximately 0.323 log higher. Spearman correlation analysis indicated that the overall changes in patient viral load patterns were highly correlative between the two assays: r = 0.912, P < 0.0001. The lower limits of sensitivity were determined to be approximately 100 copies/ml and 1,200 to 1,400 copies/ml for the NucliSens and Quantiplex assays, respectively.
摘要
本研究比较了采用Quantiplex分支DNA法和NucliSens基于核酸序列扩增法检测血浆中1型人类免疫缺陷病毒RNA的水平。通过Quantiplex法在184份样本中的118份（64.13%）检测到了RNA，通过NucliSens法在184份样本中的171份（92.94%）检测到了RNA。回归分析表明，两组数值之间存在线性关系（P<0.0001），尽管Quantiplex法和NucliSens法的数值存在显著差异（P<0.001），NucliSens法的数值大约高0.323个对数。Spearman相关性分析表明，两种检测方法在患者病毒载量模式的总体变化上高度相关：r=0.912，P<0.0001。NucliSens法和Quantiplex法的检测灵敏度下限分别确定为约100拷贝/毫升和1200至1400拷贝/毫升。
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2
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3
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9
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10
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