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评估用于定量血浆中1型人类免疫缺陷病毒RNA水平的Nuclisens HIV-1 QT检测法。

Evaluation of the Nuclisens HIV-1 QT assay for quantitation of human immunodeficiency virus type 1 RNA levels in plasma.

作者信息

Segondy M, Ly T D, Lapeyre M, Montes B

机构信息

Laboratoire de Virologie, Centre Hospitalier Universitaire de Montpellier, Montpellier, France.

出版信息

J Clin Microbiol. 1998 Nov;36(11):3372-4. doi: 10.1128/JCM.36.11.3372-3374.1998.

DOI:10.1128/JCM.36.11.3372-3374.1998
PMID:9774598
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105334/
Abstract

Nuclisens HIV-1 QT is a new version of the NASBA HIV-1 QT assay for quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma. The specificity of this assay was 100% in one laboratory and 99%-with nonrepeatability of the initial false positive-in another. The test was linear between 2.0 and 6.0 log RNA copies per ml. According to the input HIV-1 RNA concentration, accuracy varied from -0.11 to +0.10 log RNA copy per ml and precision varied from 0.66 to 0.14 log RNA copy per ml. Reproducibility decreased when the HIV-1 RNA level was near the lower limit of quantitation of the test. HIV-1 RNA could be quantitated by Nuclisens HIV-1 QT in 36% (laboratory 1) and 24% (laboratory 2) of clinical samples with HIV-1 RNA levels lower than the lower limit of quantitation by NASBA HIV-1 QT. Nuclisens HIV-1 QT was not suitable for measurement of RNA from clade G and group O HIV-1 strains.

摘要

Nuclisens HIV-1 QT是用于定量检测血浆中人类免疫缺陷病毒1型（HIV-1）RNA的NASBA HIV-1 QT检测法的新版本。该检测法在一个实验室中的特异性为100%，在另一个实验室中为99%（初始假阳性结果不可重复）。该检测在每毫升2.0至6.0 log RNA拷贝数之间呈线性关系。根据输入的HIV-1 RNA浓度，准确度在每毫升-0.11至+0.10 log RNA拷贝数之间变化，精密度在每毫升0.66至0.14 log RNA拷贝数之间变化。当HIV-1 RNA水平接近检测定量下限的时候，重现性会降低。在HIV-1 RNA水平低于NASBA HIV-1 QT定量下限的临床样本中，Nuclisens HIV-1 QT能够对36%（实验室1）和24%（实验室2）的样本进行HIV-1 RNA定量。Nuclisens HIV-1 QT不适用于检测G亚型和O组HIV-1毒株的RNA。