Peltonen R, Nenonen M, Helve T, Hänninen O, Toivanen P, Eerola E
Department of Medicine, Turku University Central Hospital, Finland.
Br J Rheumatol. 1997 Jan;36(1):64-8. doi: 10.1093/rheumatology/36.1.64.
To clarify the role of the faecal flora in the diet-induced decrease of rheumatoid arthritis (RA) activity, 43 RA patients were randomized into two groups: the test group to receive living food, a form of uncooked vegan diet rich in lactobacilli, and the control group to continue their ordinary omnivorous diets. Based on clinical assessments before, during and after the intervention period, a disease improvement index was constructed for each patient. According to the index, patients were assigned either to a group with a high improvement index (HI) or to a group with a low improvement index (LO). Stool samples collected from each patient before the intervention and at 1 month were analysed by direct stool sample gas-liquid chromatography of bacterial cellular fatty acids. This method has proved to be a simple and sensitive way to detect changes and differences in the faecal microbial flora between individual stool samples or groups of them. A significant, diet-induced change in the faecal flora (P = 0.001) was observed in the test group, but not in the control group. Further, in the test group, a significant (P = 0.001) difference was detected between the HI and LO categories at 1 month, but not in the pre-test samples. We conclude that a vegan diet changes the faecal microbial flora in RA patients, and changes in the faecal flora are associated with improvement in RA activity.
为阐明粪便菌群在饮食诱导的类风湿关节炎（RA）活动度降低中的作用，43例RA患者被随机分为两组：试验组接受生食，即一种富含乳酸菌的未烹饪纯素饮食，对照组继续其普通杂食饮食。基于干预期前后及期间的临床评估，为每位患者构建了疾病改善指数。根据该指数，患者被分为高改善指数（HI）组或低改善指数（LO）组。对每位患者在干预前及干预1个月时采集的粪便样本进行细菌细胞脂肪酸直接粪便样本气-液色谱分析。该方法已被证明是检测单个粪便样本或其组间粪便微生物菌群变化和差异的一种简单且灵敏的方法。试验组观察到饮食诱导的粪便菌群有显著变化（P = 0.001），而对照组未观察到。此外，在试验组中，干预1个月时HI和LO类别之间检测到显著差异（P = 0.001），但在试验前样本中未检测到。我们得出结论，纯素饮食可改变RA患者的粪便微生物菌群，且粪便菌群的变化与RA活动度的改善相关。
