Eerola E, Möttönen T, Hannonen P, Luukkainen R, Kantola I, Vuori K, Tuominen J, Toivanen P
Department of Medical Microbiology, Turku University, Finland.
Br J Rheumatol. 1994 Nov;33(11):1030-8. doi: 10.1093/rheumatology/33.11.1030.
To study the role of intestinal flora in the pathogenesis of RA, we have applied computerized gas-liquid chromatography (GLC) for bacterial cellular fatty acids (CFAs) present in the stool. The CFA spectra represents the total composition of bacterial CFAs in a faecal sample. Correlation and cluster analysis of CFA spectra gathers samples with quantitatively and qualitatively similar bacterial flora into clusters, which then reveal the relationship of samples to each other. Stool samples were collected at the time of hospital admission from patients with early RA before any specific treatment. The CFA spectra in stool samples of RA patients were significantly different from those of non-RA controls. Patients with erosive RA formed a group most clearly different from the controls. Analyses based on the CFA composition of reference bacteria revealed that anaerobic bacteria are primarily responsible for the differences observed. These results suggest that intestinal bacteria play a role in the development of RA.
为研究肠道菌群在类风湿关节炎（RA）发病机制中的作用，我们应用计算机化气液色谱法（GLC）分析粪便中细菌细胞脂肪酸（CFA）。CFA谱代表粪便样本中细菌CFA的总体组成。CFA谱的相关性和聚类分析将具有定量和定性相似细菌菌群的样本聚集到簇中，从而揭示样本之间的相互关系。在医院收治早期RA患者且未进行任何特异性治疗时采集粪便样本。RA患者粪便样本中的CFA谱与非RA对照者的显著不同。侵蚀性RA患者形成了与对照组差异最为明显的一组。基于参考细菌CFA组成的分析表明，厌氧菌是观察到的差异的主要原因。这些结果提示肠道细菌在RA的发生发展中起作用。
