患有慢性阻塞性肺疾病马匹的气管吸出物中的胶原酶活性及其对强力霉素抑制的敏感性。
Collagenolytic activity and its sensitivity to doxycycline inhibition in tracheal aspirates of horses with chronic obstructive pulmonary disease.
作者信息
Koivunen A L, Maisi P, Konttinen Y T, Prikk K, Sandholm M
机构信息
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland.
出版信息
Acta Vet Scand. 1997;38(1):9-16. doi: 10.1186/BF03548503.
Abstract
The collagenolytic activity and its sensitivity to doxycycline inhibition in tracheal aspirates (TA) of horses with chronic obstructive pulmonary disease (COPD) was analyzed with SDS-PA gel electrophoresis (SDS-PAGE), using Type 1 collagen as the substrate. Both autoactive and total collagenase activities were significantly higher in TAs of horses with symptomatic COPD than in TAs of healthy horses. Doxycycline inhibition studies suggest that most of the TA collagenase is of the neutrophil type (MMP-8), but some is derived from other cells such as fibroblasts and monocyte/macrophages (MMP-1) and bacteria (bacterial collagenases). Drugs inhibiting collagenases in the respiratory tract might be worth a trial in the treatment of COPD in horses.
摘要
以I型胶原蛋白为底物，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析了患有慢性阻塞性肺疾病（COPD）马匹的气管吸出物（TA）中的胶原酶活性及其对强力霉素抑制的敏感性。有症状的COPD马匹的TA中，自身活性和总胶原酶活性均显著高于健康马匹的TA。强力霉素抑制研究表明，大多数TA胶原酶属于中性粒细胞类型（基质金属蛋白酶-8，MMP-8），但有些来源于其他细胞，如成纤维细胞和单核细胞/巨噬细胞（基质金属蛋白酶-1，MMP-1）以及细菌（细菌胶原酶）。抑制呼吸道胶原酶的药物可能值得尝试用于治疗马匹的COPD。
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本文引用的文献
1
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2
3
Doxycycline in the protection of serum alpha-1-antitrypsin from human neutrophil collagenase and gelatinase.强力霉素对人中性粒细胞胶原酶和明胶酶所致血清α-1-抗胰蛋白酶的保护作用。
Antimicrob Agents Chemother. 1993 Mar;37(3):592-4. doi: 10.1128/AAC.37.3.592.
4
Reduction of matrix metalloproteinase 8-neutrophil collagenase levels during long-term doxycycline treatment of reactive arthritis.长期强力霉素治疗反应性关节炎期间基质金属蛋白酶8（中性粒细胞胶原酶）水平的降低
Antimicrob Agents Chemother. 1994 Feb;38(2):400-2. doi: 10.1128/AAC.38.2.400.
5
Collagenase in Sjögren's syndrome.干燥综合征中的胶原酶
Ann Rheum Dis. 1994 Dec;53(12):836-9. doi: 10.1136/ard.53.12.836.
6
Human neutrophil collagenase (MMP-8), identified in bronchiectasis BAL fluid, correlates with severity of disease.在支气管扩张患者的支气管肺泡灌洗液中发现的人类中性粒细胞胶原酶（MMP-8）与疾病严重程度相关。
Chest. 1995 Jun;107(6):1641-7. doi: 10.1378/chest.107.6.1641.
7
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8
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