Koivunen A L, Maisi P, Konttinen Y T, Prikk K, Sandholm M
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland.
Acta Vet Scand. 1997;38(1):9-16. doi: 10.1186/BF03548503.
The collagenolytic activity and its sensitivity to doxycycline inhibition in tracheal aspirates (TA) of horses with chronic obstructive pulmonary disease (COPD) was analyzed with SDS-PA gel electrophoresis (SDS-PAGE), using Type 1 collagen as the substrate. Both autoactive and total collagenase activities were significantly higher in TAs of horses with symptomatic COPD than in TAs of healthy horses. Doxycycline inhibition studies suggest that most of the TA collagenase is of the neutrophil type (MMP-8), but some is derived from other cells such as fibroblasts and monocyte/macrophages (MMP-1) and bacteria (bacterial collagenases). Drugs inhibiting collagenases in the respiratory tract might be worth a trial in the treatment of COPD in horses.
以I型胶原蛋白为底物，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析了患有慢性阻塞性肺疾病（COPD）马匹的气管吸出物（TA）中的胶原酶活性及其对强力霉素抑制的敏感性。有症状的COPD马匹的TA中，自身活性和总胶原酶活性均显著高于健康马匹的TA。强力霉素抑制研究表明，大多数TA胶原酶属于中性粒细胞类型（基质金属蛋白酶-8，MMP-8），但有些来源于其他细胞，如成纤维细胞和单核细胞/巨噬细胞（基质金属蛋白酶-1，MMP-1）以及细菌（细菌胶原酶）。抑制呼吸道胶原酶的药物可能值得尝试用于治疗马匹的COPD。
