Inoue M, Minami M, Fujii Y, Matsuda H, Shirakura R, Kido T
Division of Organ Transplantation, Osaka University Medical School, Japan.
J Surg Oncol. 1997 Apr;64(4):347-50. doi: 10.1002/(sici)1096-9098(199704)64:4<347::aid-jso18>3.0.co;2-#.
We describe a case of granulocyte colony-stimulating factor (GCSF) and interleukin-6 (IL-6)-producing lung cancer.
A 53-year-old man underwent left upper lobectomy under diagnosis of lung cancer. The tumor obtained by a preoperative biopsy was analyzed.
Preoperative data showed leukocytosis with left-shift of leukocytic morphology and thrombocytosis and an elevated serum GCSF level. Histological examination revealed poorly differentiated adenocarcinoma. A cell line, named LCAM, was established from the tumor and the cytokines in the culture medium were measured by enzyme immunoassay. GCSF and IL-6 were produced in large amounts by LCAM, but granulocyte-macrophage colony-stimulating factor (GMCSF) and interleukin-3 (IL-3) were not. A proportion of LCAM expressed GCSF receptor on the cell surface, but IL-6 receptor could not be detected. LCAM proliferation was inhibited in the culture with antihuman GCSF antibody in a dose-dependent manner.
We suggest that LCAM proliferation is positively regulated by GCSF.
我们描述了一例产生粒细胞集落刺激因子（GCSF）和白细胞介素-6（IL-6）的肺癌病例。
一名53岁男性在肺癌诊断下行左上肺叶切除术。对术前活检获取的肿瘤进行分析。
术前数据显示白细胞增多伴白细胞形态左移、血小板增多以及血清GCSF水平升高。组织学检查显示为低分化腺癌。从肿瘤中建立了一个名为LCAM的细胞系，并通过酶免疫测定法检测培养基中的细胞因子。LCAM大量产生GCSF和IL-6，但不产生粒细胞-巨噬细胞集落刺激因子（GMCSF）和白细胞介素-3（IL-3）。一部分LCAM在细胞表面表达GCSF受体，但未检测到IL-6受体。在含有抗人GCSF抗体的培养物中，LCAM的增殖以剂量依赖方式受到抑制。
我们认为LCAM的增殖受到GCSF的正向调节。
