Nikawa H, Yamamoto T, Hamada T, Rahardjo M B, Murata H, Nakanoda S
Department of Prosthetic Dentistry, Hiroshima University School of Dentistry, Minami-ku, Japan.
J Oral Rehabil. 1997 May;24(5):350-7. doi: 10.1046/j.1365-2842.1997.d01-297.x.
A new antimicrobial material, Ag-zeolite (Zeomic), was combined with a commercial tissue conditioner (GC-Soft Liner (GC); 1-5%) and, through monitoring the pH of the growth medium, examined for effects on the in vitro growth and/or acid production of Candida albicans on protein-free and saliva-coated specimens. The effect of incorporation of this agent on the physical property of the lining material was also examined according to the ISO penetration test. Comparison studies were carried out using GC, Coe Comfort (CC) or undecylenate combined GC (1-5%) specimens. Although the pH changes in the media varied depending upon the materials on which the Candida was grown, reverse sigmoidal pH curves were observed with most samples. As compared with GC, the soft lining materials showed, to some extent, an inhibitory effect on the acid production and/or the growth of C. albicans. These inhibitory effects consisted of a delay in the onset of rapid pH decline, decreases in the rate of pH change and increases in minimum pH. In most cases, the inhibitory effects of test specimens were dose-dependent, and zeolite specimens showed a significantly higher antifungal effect, followed by CC and undecylenate-combined GC; GC showed the least antifungal effect. The inhibitory effects of these materials on fungal growth were decreased by the presence of a saliva-coat, particularly with zeolite specimens and CC. However, four of eight 5%-Zeomic specimens still exhibited perfect growth inhibition in the presence of the salivary pellicle. Furthermore, test specimens containing 2-5% Zeomic showed a significantly greater effect on the delay in rapid decline of pH, as compared with the other specimens examined. In addition, the significantly higher minimum pH was observed where the yeasts were grown on 4%- and 5%-Zeomic specimens. The physical properties of all the test specimens conformed with the ISO standard as examined by penetration test. These results taken together suggest that an antimicrobial zeolite-combined tissue conditioner would be a potential aid in denture plaque control.
一种新型抗菌材料银沸石（Zeomic）与一种市售组织调理剂（GC-Soft Liner（GC）；1%-5%）相结合，并通过监测生长培养基的pH值，研究其对白色念珠菌在无蛋白和唾液包被标本上的体外生长和/或产酸的影响。还根据ISO渗透试验研究了该制剂掺入对衬里材料物理性能的影响。使用GC、Coe Comfort（CC）或十一烯酸盐联合GC（1%-5%）标本进行了比较研究。尽管培养基中的pH变化因念珠菌生长的材料而异，但大多数样品观察到反向S形pH曲线。与GC相比，软衬里材料在一定程度上对白色念珠菌的产酸和/或生长具有抑制作用。这些抑制作用包括快速pH下降开始的延迟、pH变化速率降低和最低pH升高。在大多数情况下，测试标本的抑制作用呈剂量依赖性，沸石标本显示出显著更高的抗真菌效果，其次是CC和十一烯酸盐联合GC；GC显示出最低的抗真菌效果。这些材料对真菌生长的抑制作用因唾液包被的存在而降低，尤其是沸石标本和CC。然而，八个5%-Zeomic标本中的四个在存在唾液膜的情况下仍表现出完美的生长抑制。此外，与其他测试标本相比，含有2%-5% Zeomic的测试标本对pH快速下降的延迟有显著更大的影响。此外，在4%-和5%-Zeomic标本上生长酵母的地方观察到显著更高的最低pH。通过渗透试验检查，所有测试标本的物理性能均符合ISO标准。综合这些结果表明，一种抗菌沸石联合组织调理剂可能有助于控制假牙菌斑。
