转化生长因子-β基因家族成员生长分化因子5、6和7在大鼠体内异位诱导肌腱和韧带生成
Ectopic induction of tendon and ligament in rats by growth and differentiation factors 5, 6, and 7, members of the TGF-beta gene family.
作者信息
Wolfman N M, Hattersley G, Cox K, Celeste A J, Nelson R, Yamaji N, Dube J L, DiBlasio-Smith E, Nove J, Song J J, Wozney J M, Rosen V
机构信息
Genetics Institute, Inc., Cambridge, Massachusetts 02140, USA.
出版信息
J Clin Invest. 1997 Jul 15;100(2):321-30. doi: 10.1172/JCI119537.
Abstract
Little is known about the regulatory signals involved in tendon and ligament formation, and this lack of understanding has hindered attempts to develop biologically based therapies for tendon and ligament repair. Here we report that growth and differentiation factors (GDFs) 5, 6, and 7, members of the TGF-beta gene superfamily that are most related to the bone morphogenetic proteins, induce neotendon/ligament formation when implanted at ectopic sites in vivo. Analysis of tissue induced by GDF-5, 6, or 7, containing implants by currently available morphological and molecular criteria used to characterize tendon and ligament, adds further evidence to the idea that these GDFs act as signaling molecules during embryonic tendon/ligament formation. In addition, comparative in situ localizations of the GDF-5, 6, and 7 mRNAs suggest that these molecules are important regulatory components of synovial joint morphogenesis.
摘要
关于肌腱和韧带形成过程中涉及的调控信号，人们了解甚少，而这种认知的不足阻碍了开发基于生物学的肌腱和韧带修复疗法的尝试。在此我们报告，生长分化因子（GDFs）5、6和7，即TGF-β基因超家族中与骨形态发生蛋白关系最为密切的成员，当在体内异位植入时可诱导新肌腱/韧带形成。通过目前用于表征肌腱和韧带的形态学和分子标准对含有GDF-5、6或7植入物所诱导的组织进行分析，进一步证明了这些GDFs在胚胎肌腱/韧带形成过程中作为信号分子发挥作用。此外，GDF-5、6和7 mRNA的比较原位定位表明，这些分子是滑膜关节形态发生的重要调控成分。
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本文引用的文献
1
Functional cooperation between the non-paralogous genes Hoxa-10 and Hoxd-11 in the developing forelimb and axial skeleton.非同源基因Hoxa - 10和Hoxd - 11在发育中的前肢和轴骨骼中的功能协作。
Development. 1996 Feb;122(2):449-60. doi: 10.1242/dev.122.2.449.
2
Transient introduction of a foreign gene into healing rat patellar ligament.将外源基因短暂导入愈合中的大鼠髌韧带。
J Clin Invest. 1996 Jan 1;97(1):226-31. doi: 10.1172/JCI118395.
3
Proteoglycans in the compressed region of human tibialis posterior tendon and in ligaments.人胫后肌腱压缩区域和韧带中的蛋白聚糖。
J Orthop Res. 1993 Jan;11(1):68-77. doi: 10.1002/jor.1100110109.
4
Limb alterations in brachypodism mice due to mutations in a new member of the TGF beta-superfamily.由于TGF-β超家族一个新成员发生突变导致短肢小鼠的肢体改变。
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5
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6
7
The effect of combinations of growth factors on ligament healing.生长因子组合对韧带愈合的影响。
Clin Orthop Relat Res. 1994 Nov(308):207-12.
8
Stress governs tissue phenotype at the femoral insertion of the rabbit MCL.应力决定兔内侧副韧带股骨附着处的组织表型。
J Biomech. 1995 Feb;28(2):147-57. doi: 10.1016/0021-9290(94)00058-c.
9
Dorsalizing signal Wnt-7a required for normal polarity of D-V and A-P axes of mouse limb.背侧化信号Wnt-7a是小鼠肢体背腹和前后轴正常极性所必需的。
Nature. 1995 Mar 23;374(6520):350-3. doi: 10.1038/374350a0.
10
Elastin exhibits a distinctive temporal and spatial pattern of distribution in the developing chick limb in association with the establishment of the cartilaginous skeleton.随着软骨骨骼的形成，弹性蛋白在发育中的鸡肢体中呈现出独特的时间和空间分布模式。
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