Bobkov A, Cheingsong-Popov R, Selimova L, Ladnaya N, Kazennova E, Kravchenko A, Fedotov E, Saukhat S, Zverev S, Pokrovsky V, Weber J
The D.I. Ivanovsky Institute of Virology, Moscow, Russia.
AIDS Res Hum Retroviruses. 1997 Sep 20;13(14):1195-201. doi: 10.1089/aid.1997.13.1195.
Epidemiological data have demonstrated rapid growth of HIV-1 infections among injecting drug users (IDUs) in the Ukraine and Russia, during 1996. Here we describe the results of genetic analysis of isolates derived from 12 HIV-1-infected IDUs in different sites of Russia and the Ukraine. The blood samples were taken within a 1- to 2-month period after the first HIV-1-positive test. The results of the heteroduplex mobility assay as well as gag/env phylogenetic analysis reveal that all sequences belong to gag/env genetic subtype A. Moreover, interpatient genetic distances between the nucleotide sequences encompassing the C2-V3, the V4-V5, and p17-encoding regions within this group were low (the average means were 0.9, 1.3, and 0.4%, respectively). These data show a marked homogeneity of HIV-1, probably spreading during primary infection. It is possible that the current epidemic of subtype A HIV-1 among IDUs in the former Soviet Union is caused by a point source exposure.
流行病学数据显示，1996年期间，乌克兰和俄罗斯注射吸毒者（IDU）中的HIV-1感染呈快速增长态势。在此，我们描述了对来自俄罗斯和乌克兰不同地区12名HIV-1感染注射吸毒者的病毒分离株进行基因分析的结果。血样在首次HIV-1检测呈阳性后的1至2个月内采集。异源双链迁移率分析以及gag/env系统发育分析结果显示，所有序列均属于gag/env基因亚型A。此外，该组内包含C2-V3、V4-V5和p17编码区的核苷酸序列之间的患者间基因距离较低（平均分别为0.9%、1.3%和0.4%）。这些数据表明HIV-1具有显著的同质性，可能在初次感染期间传播。前苏联注射吸毒者中目前的A型HIV-1流行可能是由单点源暴露引起的。
