Rottman J B, Ganley K P, Williams K, Wu L, Mackay C R, Ringler D J
LeukoSite Inc., Cambridge, MA 02142, USA.
Am J Pathol. 1997 Nov;151(5):1341-51.
The chemokine receptor CCR5 has recently been described as a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1. In this study, using a panel of monoclonal antibodies specific for human CCR5, we show by immunohistochemistry and flow cytometry that CCR5 is expressed by bone-marrow-derived cells known to be targets for HIV-1 infection, including a subpopulation of lymphocytes and monocyte/macrophages in blood, primary and secondary lymphoid organs, and noninflamed tissues. In the central nervous system, CCR5 is expressed on neurons, astrocytes, and microglia. In other tissues, CCR5 is expressed on epithelium, endothelium, vascular smooth muscle, and fibroblasts. Chronically inflamed tissues contain an increased number of CCR5+ mononuclear cells, and the number of immunoreactive cells is directly associated with a histopathological correlate of inflammatory severity. Collectively, these results suggest that CCR5+ cells are recruited to inflammatory sites and, as such, may facilitate transmission of macrophage-tropic strains of HIV-1.
趋化因子受体CCR5最近被描述为人类免疫缺陷病毒1型（HIV-1）嗜巨噬细胞株的共受体。在本研究中，我们使用一组针对人类CCR5的单克隆抗体，通过免疫组织化学和流式细胞术表明，CCR5在已知为HIV-1感染靶标的骨髓来源细胞中表达，包括血液、一级和二级淋巴器官以及未发炎组织中的淋巴细胞亚群和单核细胞/巨噬细胞。在中枢神经系统中，CCR5在神经元、星形胶质细胞和小胶质细胞上表达。在其他组织中，CCR5在上皮细胞、内皮细胞、血管平滑肌和成纤维细胞上表达。慢性炎症组织中CCR5+单核细胞数量增加，免疫反应性细胞数量与炎症严重程度的组织病理学相关指标直接相关。总体而言，这些结果表明CCR5+细胞被招募到炎症部位，因此可能促进HIV-1嗜巨噬细胞株的传播。
