Rossi G B, Cioe L, Pulciani S, Meo P, Titti F, Amici D, Gianfranceschi G L
Mol Biol Rep. 1979 Dec 31;5(4):241-9.
Low molecular weight chromatin peptides exert a dose-dependent inhibition of Dimethylsulfoxide (DMSO)-induced erythroid differentiation of murine Friend Leukemia Cells (FLC). This effect correlates with the degree of purification of the peptide fractions. Crot analysis of globin mRNA amounts in DMSO-treated FLC given the peptides showed a 4-5-fold decrease of messenger RNA in the cytoplasma with no nuclear storage of globin transcripts. Spectrin accumulation in "induced" FLC is inhibited as well. The effects of the peptides on erythroid markers are reversible upon removal of the compounds. They also appear to be specific for induced gene expression as (1) no effects are observed on cell growth and RNA synthesis in normal non-differentiating cell lines; and (2) no changes have been detected with regard to the expression of integrated viral genes coding for continuous shedding of viral particles.
低分子量染色质肽对二甲基亚砜（DMSO）诱导的小鼠Friend白血病细胞（FLC）红系分化具有剂量依赖性抑制作用。这种效应与肽组分的纯化程度相关。对给予肽的DMSO处理的FLC中的珠蛋白mRNA量进行的Crot分析显示，细胞质中信使RNA减少了4至5倍，且珠蛋白转录本无核内储存。“诱导”的FLC中血影蛋白的积累也受到抑制。去除这些化合物后，肽对红系标志物的作用是可逆的。它们似乎对诱导的基因表达具有特异性，因为（1）在正常未分化细胞系中未观察到对细胞生长和RNA合成的影响；（2）对于编码病毒颗粒持续释放的整合病毒基因的表达未检测到变化。
