Nguyen S V, To H, Minamoto N, Ogawa M, Yamaguchi T, Fukushi H, Hirai K
Department of Veterinary Microbiology, Gifu University, Japan.
Clin Diagn Lab Immunol. 1997 Nov;4(6):676-80. doi: 10.1128/cdli.4.6.676-680.1997.
The usefulness of the high-density particle agglutination (HDPA) test as a potential tool for the detection of anti-Coxiella burnetii antibodies in animal sera was studied by using 619 cow, 589 dog, and 150 cat serum samples and antisera from rabbits, guinea pigs, and mice. The sensitivity and specificity of the test versus those of the reference microimmunofluorescence test were determined at two different threshold titer values. At the cutoff value of 1:16, the sensitivities of the HDPA test for cow, dog, and cat sera were 94.3, 95, and 91.3%, respectively, and the specificities were 95.5, 95.3, and 91.3%, respectively. At the cutoff value of 1:32, the sensitivities were 86.7, 88.3, and 82.6%, respectively, and the specificities were 99, 99.2, and 98.4%, respectively. For the group of immune laboratory animals all samples from rabbits, guinea pigs, and mice were positive by the HDPA test. The erythrocyte-sensitizing substance from phase II C. burnetii was found to contain protein and carbohydrate, and both fractions are immunoreactive. The study results show that the HDPA test is a useful tool in the epizootiological survey of Coxiella infection in animals.
通过使用619份牛血清、589份犬血清、150份猫血清样本以及兔、豚鼠和小鼠的抗血清，研究了高密度颗粒凝集（HDPA）试验作为检测动物血清中抗伯氏考克斯氏体抗体的潜在工具的实用性。在两个不同的临界滴度值下，测定了该试验相对于参考微量免疫荧光试验的敏感性和特异性。在截断值为1:16时，HDPA试验对牛、犬和猫血清的敏感性分别为94.3%、95%和91.3%，特异性分别为95.5%、95.3%和91.3%。在截断值为1:32时，敏感性分别为86.7%、88.3%和82.6%，特异性分别为99%、99.2%和98.4%。对于免疫实验动物组，HDPA试验显示来自兔、豚鼠和小鼠的所有样本均为阳性。发现来自第二阶段伯氏考克斯氏体的红细胞致敏物质含有蛋白质和碳水化合物，且这两种成分均具有免疫反应性。研究结果表明，HDPA试验是动物伯氏考克斯氏体感染流行病学调查中的一种有用工具。
