Benítez T, Villa T G, Acha I G
Arch Microbiol. 1975 Nov 7;105(3):277-82. doi: 10.1007/BF00447147.
When incubated in Winge medium, protoplasts from Trichoderma viride obtained by treatment with Micromonospora chalcea or Streptomyces venezuelae RA lytic systems first synthesized an aberrant wall, different from the normal one; it was aseptate, larger and irregular in size and length. They then regenerated a new wall, similar to the original one from which they were liberated. Analysis showed that the wall polymers were mainly beta-(1-3) glucan, beta-(1-6) glucan and chitin in the normal walls, whereas chitin was absent in aberrant tubes. These results are discussed below together with electron micrographs of aberrant and normal walls.
在温格培养基中培养时，用淡紫小单孢菌或委内瑞拉链霉菌RA裂解系统处理获得的绿色木霉原生质体首先合成了一种异常壁，与正常壁不同；它无隔膜，尺寸和长度更大且不规则。然后它们再生出一个新壁，类似于它们从中释放出来的原始壁。分析表明，正常壁中的壁聚合物主要是β-(1-3)葡聚糖、β-(1-6)葡聚糖和几丁质，而异常管中不存在几丁质。下面将结合异常壁和正常壁的电子显微照片讨论这些结果。
