Kuhn M
Theodor-Boveri-Institut für Biowissenschaften, Universität Würzburg, Lehrstuhl für Mikrobiologie, Germany.
FEMS Microbiol Lett. 1998 Mar 1;160(1):87-90. doi: 10.1111/j.1574-6968.1998.tb12895.x.
Uptake of Listeria monocytogenes by different mammalian cells like macrophages and epithelial cells is dependent on functional actin filaments and hence susceptible to inhibition by cytochalasin. Here we show that phagocytic uptake of L. monocytogenes by P388D1 macrophages is also highly sensitive to treatment with the microtubule depolymerizing drugs nocodazole and colchicine. This sensitivity is cell type specific and much less pronounced in bone marrow-derived macrophages and Caco-2 epithelial cells. In contrast to nocodazole and colchicine, the microtubule stabilizing drug taxol has no significant effect on the uptake of L. monocytogenes by all three cell types tested.
不同的哺乳动物细胞（如巨噬细胞和上皮细胞）对单核细胞增生李斯特菌的摄取依赖于功能性肌动蛋白丝，因此易受细胞松弛素的抑制。在此我们表明，P388D1巨噬细胞对单核细胞增生李斯特菌的吞噬摄取对微管解聚药物诺考达唑和秋水仙碱的处理也高度敏感。这种敏感性具有细胞类型特异性，在骨髓来源的巨噬细胞和Caco-2上皮细胞中则不太明显。与诺考达唑和秋水仙碱不同，微管稳定药物紫杉醇对所测试的所有三种细胞类型摄取单核细胞增生李斯特菌均无显著影响。
