Wetzel W, Ott T, Matthies H
Pharmacol Biochem Behav. 1976 May;4(5):515-9. doi: 10.1016/0091-3057(76)90190-8.
The influence of Actinomycin D (AMD) applied intrahippocampally at doses of 1-6 mug/animal, on the acquisition and retention of a shock-motivated brightness discrimination was studied on rats in a semiautomatic Y-maze. The injection of AMD 4 hr prior to training did not influence the acquisition, but causes, dose-dependent, a retention loss in relearning 24 hr after training. Twenty-eight hr after AMD application, naive rats exhibited a deterioration of acquisition performance increasing equally with the dose. At the same time, both circumscribed necroses in the hippocampus and signs of a general intoxication were observed. Considering the described pro- and retroactive effects, it is concluded that the use of the inhibitor AMD in learning experiments is not suitable to provide reliable evidence of the specific importance of the cerebral RNA synthesis for memory consolidation.
在半自动Y型迷宫中，研究了以1 - 6微克/动物的剂量海马内注射放线菌素D（AMD）对大鼠电击驱动的亮度辨别学习和记忆保持的影响。训练前4小时注射AMD不影响学习，但会导致训练后24小时再学习时出现剂量依赖性的记忆丧失。AMD注射后28小时，未训练的大鼠表现出学习能力下降，且与剂量呈正比。同时，观察到海马中有局限性坏死以及全身中毒的迹象。考虑到所描述的前摄和追溯效应，得出结论：在学习实验中使用抑制剂AMD并不适合为大脑RNA合成对记忆巩固的特定重要性提供可靠证据。
