Paré J A, Sigler L, Hunter D B, Summerbell R C, Smith D A, Machin K L
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
J Zoo Wildl Med. 1997 Dec;28(4):443-53.
A dermatophyte-like fungus was isolated from skin biopsies of three different species of captive chameleon in which fungal elements had been observed by histologic examination. An adult Parson's chameleon (Chamaeleo parsonii) presented with vesicles that became crusty brown lesions on the limbs and body. Skin biopsies revealed fungal hyphae in the affected epidermis and underlying dermis. The lesions regressed fully after oral administration of itraconazole. An adult jewel chameleon (Chamaeleo lateralis) from the same private collection presented with localized black skin lesions and died while being treated with itraconazole. A pulmonary granuloma was also present in this chameleon at autopsy. Cultures obtained from skin and lung lesions yielded the same fungus. A third isolate was obtained from a skin biopsy of a Jackson's chameleon (Chamaeleo jacksoni) with deep ulcerative cutaneous lesions located at the base of the tail. The fungus, in all three cases, has been identified as the Chrysosporium anamorph of Nannizziopsis vriesii, a poorly known ascomycetous species recorded previously from the skin of a lizard and from soil, on the basis of its keratinolytic activity, resistance to cycloheximide, strongly restricted growth at 37 degrees C, formation of clavate or pyriform single-celled or two-celled aleurioconidia, and alternate and fission arthroconidia.
从三只圈养变色龙的皮肤活检样本中分离出一种类似皮肤癣菌的真菌，经组织学检查已观察到真菌成分。一只成年的帕森斯变色龙（Chamaeleo parsonii）四肢和身体出现水疱，后变成结痂的褐色病变。皮肤活检显示，受影响的表皮和真皮中有真菌菌丝。口服伊曲康唑后，病变完全消退。同一家私人收藏中的一只成年宝石变色龙（Chamaeleo lateralis）出现局部黑色皮肤病变，在接受伊曲康唑治疗时死亡。尸检发现这只变色龙还有肺部肉芽肿。从皮肤和肺部病变处获取的培养物培养出相同的真菌。第三个分离株是从一只杰克逊变色龙（Chamaeleo jacksoni）的皮肤活检样本中获得的，该变色龙尾巴根部有深部溃疡性皮肤病变。基于其角蛋白分解活性、对放线菌酮的抗性、在37摄氏度下生长强烈受限、形成棒状或梨形单细胞或双细胞粉孢子以及交替和裂生关节孢子，在所有三个病例中，该真菌均被鉴定为弗里西拟南芥金孢子菌，这是一种此前在蜥蜴皮肤和土壤中记录过的鲜为人知的子囊菌物种。
