Lafon M E, Dutronc H, Dubois V, Pellegrin I, Barbeau P, Ragnaud J M, Pellegrin J L, Fleury H J
Laboratoire de Virologie, Institut Fédératif de Recherches en Pathologies Infectieuses, Université Bordeaux 2, France.
J Infect Dis. 1998 Jun;177(6):1502-5. doi: 10.1086/515305.
JC virus (JCV) is thought to reach the central nervous system by a vascular route. To determine whether JCV is conveyed in peripheral blood as latent or reactivated virus, blood leukocytes, plasma, and urine from 50 AIDS patients and plasma and B lymphocytes from 60 AIDS patients were investigated. Peripheral blood from 88 human immunodeficiency virus-negative blood donors was studied. Nested polymerase chain reaction assays allowed the identification of JCV T DNA and VP1 mRNAs. The latter indicate viral replication. Blood harbored JCV DNA in 31.8% of AIDS patients (only 2.3% of blood donors; P > .001) and urine in 56%. VP1 mRNAs were detected in blood of 1 AIDS patient. Notably, 38% of DNA-positive urine samples and 10 cerebrospinal fluid samples (CSF) from AIDS patients with progressive multifocal leukoencephalopathy contained JCV mRNAs. Thus, JCV was significantly more frequent in blood from AIDS patients than from controls, but, in most instances, it was latent, whereas active replication was detected in urine and CSF.
人们认为JC病毒（JCV）通过血管途径进入中枢神经系统。为了确定JCV是以潜伏病毒还是再激活病毒的形式在外周血中传播，对50例艾滋病患者的血液白细胞、血浆和尿液以及60例艾滋病患者的血浆和B淋巴细胞进行了研究。对88名人类免疫缺陷病毒阴性献血者的外周血也进行了研究。巢式聚合酶链反应检测可鉴定JCV T DNA和VP1 mRNA。后者表明病毒正在复制。31.8%的艾滋病患者血液中含有JCV DNA（献血者中仅为2.3%；P>.001），56%的尿液中含有JCV DNA。在1例艾滋病患者的血液中检测到VP1 mRNA。值得注意的是，38%的DNA阳性尿液样本以及来自患有进行性多灶性白质脑病的艾滋病患者的10份脑脊液样本中含有JCV mRNA。因此，JCV在艾滋病患者血液中的出现频率显著高于对照组，但在大多数情况下，它处于潜伏状态，而在尿液和脑脊液中检测到了活跃复制。
