Takahashi Y, Murai C, Shibata S, Munakata Y, Ishii T, Ishii K, Saitoh T, Sawai T, Sugamura K, Sasaki T
Department of Clinical and Laboratory Medicine, Tohoku University School of Medicine, Sendai, 980-8574 Japan.
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8227-32. doi: 10.1073/pnas.95.14.8227.
Human parvovirus B19 (B19) DNA was detected in the synovial tissues in 30 of 39 patients with rheumatoid arthritis (RA), and infrequently in those with osteoarthritis and traumatic joints. On the other hand, the expression of the B19 antigen VP-1 was specific (27/27) in RA synovium with active synovial lesions, but not in osteoarthritis and controls. The target cells of B19 were macrophages, follicular dendritic cells, T cells, and B cells, but not synovial lining cells in the synovium. B19-negative bone marrow cells, tonsil cells, and macrophage cell line U-937 cells became positive for the expression of VP-1, and more productive for interleukin 6 and tumor necrosis factor alpha when cocultured with RA synovial cells. The expression of VP-1 and the production of interleukin 6 and tumor necrosis factor alpha was significantly inhibited by the addition of neutralizing antibody for B19, suggesting that B19 detected in RA synovial cells is infective. B19 is involved in the initiation and perpetuation of RA synovitis, leading to joint lesions.
在39例类风湿关节炎（RA）患者中，30例患者的滑膜组织检测到人类细小病毒B19（B19）DNA，而骨关节炎和创伤性关节患者中则很少检测到。另一方面，B19抗原VP-1的表达在有活跃滑膜病变的RA滑膜中具有特异性（27/27），而在骨关节炎和对照组中则无。B19的靶细胞是巨噬细胞、滤泡树突状细胞、T细胞和B细胞，而非滑膜中的滑膜衬里细胞。B19阴性的骨髓细胞、扁桃体细胞和巨噬细胞系U-937细胞与RA滑膜细胞共培养时，VP-1表达呈阳性，白细胞介素6和肿瘤坏死因子α的产生也更多。添加B19中和抗体可显著抑制VP-1的表达以及白细胞介素6和肿瘤坏死因子α的产生，这表明在RA滑膜细胞中检测到的B19具有感染性。B19参与RA滑膜炎的起始和持续，导致关节病变。
