Aguilar C, Pujol I, Sala J, Guarro J
Unitat de Microbiologia, Facultat de Medicina, Universitat Rovira i Virgili, Reus, Tarragona, Spain.
Antimicrob Agents Chemother. 1998 Jul;42(7):1601-4. doi: 10.1128/AAC.42.7.1601.
The MICs and minimum fungicidal concentrations (MFCs) of amphotericin B, miconazole, itraconazole, ketoconazole, fluconazole, and flucytosine for 52 isolates of Paecilomyces species were evaluated by the broth microdilution method, largely based on the recommendations of the National Committee for Clinical Laboratory Standards (document M27-A). The fungal isolates tested included 16 P. variotii, 11 P. lilacinus, 9 P. marquandii, 6 P. fumosoroseus, 4 P. javanicus, and 2 P. viridis isolates and 1 isolate of each of the following species: P. carneus, P. farinosus, P. fulvus, and P. niveus. The MFCs and the MICs at which 90% of isolates were inhibited (MIC90s) for the six antifungal agents were remarkably high; the MIC50s indicated that amphotericin B, miconazole, itraconazole, and ketoconazole had good activities, while fluconazole and flucytosine demonstrated poor efficacy. The ranges of the MICs were generally wider and lower than those of the MFCs. There were significant susceptibility differences among the species. All species with the exception of P. variotii were highly resistant to fluconazole and flucytosine; P. variotii was susceptible to flucytosine. Amphotericin B and the rest of the azoles showed good activity against P. variotii, while all the antifungal agents assayed showed low efficacy against P. lilacinus.
采用肉汤微量稀释法，主要依据美国国家临床实验室标准委员会（文件M27 - A）的建议，对两性霉素B、咪康唑、伊曲康唑、酮康唑、氟康唑和氟胞嘧啶针对52株拟青霉属菌株的最低抑菌浓度（MICs）和最低杀菌浓度（MFCs）进行了评估。所测试的真菌分离株包括16株变幻拟青霉、11株淡紫拟青霉、9株马尔克拟青霉、6株玫烟色拟青霉、4株爪哇拟青霉、2株绿色拟青霉，以及以下各1株：肉色拟青霉、粉状拟青霉、黄拟青霉和雪白拟青霉。这六种抗真菌药物的MFCs以及抑制90%分离株生长的MICs（MIC90s）非常高；MIC50s表明两性霉素B、咪康唑、伊曲康唑和酮康唑活性良好，而氟康唑和氟胞嘧啶疗效较差。MICs的范围通常比MFCs更宽且更低。不同菌种之间存在显著的药敏差异。除变幻拟青霉外，所有菌种对氟康唑和氟胞嘧啶高度耐药；变幻拟青霉对氟胞嘧啶敏感。两性霉素B和其余唑类药物对变幻拟青霉显示出良好活性，而所有检测的抗真菌药物对淡紫拟青霉疗效均较低。
