通过巢式PCR直接鉴定人血清中的伯氏考克斯氏体质粒。
Direct identification of Coxiella burnetii plasmids in human sera by nested PCR.
作者信息
Zhang G Q, Hotta A, Mizutani M, Ho T, Yamaguchi T, Fukushi H, Hirai K
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
出版信息
J Clin Microbiol. 1998 Aug;36(8):2210-3. doi: 10.1128/JCM.36.8.2210-2213.1998.
Nested PCR assays were used for the direct identification of Coxiella burnetii plasmids in human sera. A total of 81 serum samples from 81 patients with Q fever were tested by nested PCR with four sets of primers. The first set of primers was used to detect the genomic sequences. The second set of primers was used to detect the conserved sequences of the plasmids. Another two sets of primers were used to identify the QpH1 and QpRS plasmids. QpH1 and QpRS plasmid-specific sequences were identified in 40 (49.4%) and 24 (29.6%) of the serum samples, respectively. Both of the QpH1 and QpRS plasmid-specific sequences were detected in 5 (8.6%) of the serum samples but were not found in 12 (20.7%) of the serum samples. Furthermore, all of the 23 acute-phase serum samples were positive for the QpH1 plasmid and negative for the QpRS plasmid. Nested PCR with plasmid-specific primers appears to be a useful method for the direct typing of C. burnetii plasmids in human sera.
巢式PCR检测用于直接鉴定人血清中的伯氏考克斯氏体质粒。使用四组引物通过巢式PCR对81例Q热患者的81份血清样本进行检测。第一组引物用于检测基因组序列。第二组引物用于检测质粒的保守序列。另外两组引物用于鉴定QpH1和QpRS质粒。分别在40份（49.4%）和24份（29.6%）血清样本中鉴定出QpH1和QpRS质粒特异性序列。在5份（8.6%）血清样本中同时检测到QpH1和QpRS质粒特异性序列，但在12份（20.7%）血清样本中未检测到。此外，所有23份急性期血清样本的QpH1质粒呈阳性，QpRS质粒呈阴性。使用质粒特异性引物的巢式PCR似乎是直接鉴定人血清中伯氏考克斯氏体质粒类型的一种有用方法。
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