烟草ndhB基因的定向破坏会损害围绕光系统I的循环电子流。
Directed disruption of the tobacco ndhB gene impairs cyclic electron flow around photosystem I.
作者信息
Shikanai T, Endo T, Hashimoto T, Yamada Y, Asada K, Yokota A
机构信息
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0101 Japan.
出版信息
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9705-9. doi: 10.1073/pnas.95.16.9705.
Abstract
To evaluate the physiological significance of cyclic electron flow around photosystem (PS) I, we used a reverse genetic approach to focus on 11 chloroplast genes that encode homologs of mitochondrial complex I subunits (ndhA-K). Since their discovery, the exact function of the respiratory components in plant chloroplasts has been a matter of discussion. We disrupted one of these genes (ndhB) in tobacco by chloroplast transformation. Analysis of the transient increase in chlorophyll fluorescence after actinic light illumination and the redox kinetics of P700 (reaction center chlorophylls of PS I) suggest that the cyclic electron flow around PS I is impaired in the ndhB-deficient transformants. Transformants grew normally in a greenhouse, suggesting that the cyclic electron flow around PS I mediated by ndh gene products is dispensable in tobacco under mild environmental conditions.
摘要
为了评估围绕光系统（PS）I的循环电子流的生理意义，我们采用反向遗传学方法，聚焦于11个叶绿体基因，这些基因编码线粒体复合体I亚基（ndhA-K）的同源物。自它们被发现以来，植物叶绿体中呼吸成分的确切功能一直是讨论的焦点。我们通过叶绿体转化破坏了烟草中的其中一个基因（ndhB）。对光化光照射后叶绿素荧光的瞬时增加以及P700（PS I的反应中心叶绿素）的氧化还原动力学分析表明，在ndhB缺陷型转化体中，围绕PS I的循环电子流受到损害。转化体在温室中正常生长，这表明在温和环境条件下，由ndh基因产物介导的围绕PS I的循环电子流在烟草中是可有可无的。
相似文献
1
Directed disruption of the tobacco ndhB gene impairs cyclic electron flow around photosystem I.烟草ndhB基因的定向破坏会损害围绕光系统I的循环电子流。
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9705-9. doi: 10.1073/pnas.95.16.9705.
2
Increased sensitivity of photosynthesis to antimycin A induced by inactivation of the chloroplast ndhB gene. Evidence for a participation of the NADH-dehydrogenase complex to cyclic electron flow around photosystem I.叶绿体ndhB基因失活导致光合作用对抗霉素A的敏感性增加。NADH脱氢酶复合体参与围绕光系统I的循环电子流的证据。
Plant Physiol. 2001 Apr;125(4):1919-29. doi: 10.1104/pp.125.4.1919.
3
Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes.通过分析含有叶绿体ndh基因破坏的烟草突变体鉴定叶绿体中的功能性呼吸复合体。
EMBO J. 1998 Feb 16;17(4):868-76. doi: 10.1093/emboj/17.4.868.
4
Study of tobacco transformants to assess the role of chloroplastic NAD(P)H dehydrogenase in photoprotection of photosystems I and II.烟草转化体研究，以评估叶绿体NAD(P)H脱氢酶在光系统I和II光保护中的作用。
Planta. 2002 Dec;216(2):273-9. doi: 10.1007/s00425-002-0843-0. Epub 2002 Aug 21.
5
Cyclic electron flow around photosystem I in C(3) plants. In vivo control by the redox state of chloroplasts and involvement of the NADH-dehydrogenase complex.C3植物中围绕光系统I的循环电子流。叶绿体氧化还原状态的体内调控及NADH脱氢酶复合体的参与。
Plant Physiol. 2002 Feb;128(2):760-9. doi: 10.1104/pp.010775.
6
Enhanced ferredoxin-dependent cyclic electron flow around photosystem I and alpha-tocopherol quinone accumulation in water-stressed ndhB-inactivated tobacco mutants.水分胁迫下ndhB失活烟草突变体中光系统I周围增强的铁氧化还原蛋白依赖性循环电子流及α-生育酚醌积累
Planta. 2005 Oct;222(3):502-11. doi: 10.1007/s00425-005-1548-y. Epub 2005 May 24.
7
A novel mechanism of nuclear photosynthesis gene regulation by redox signals from the chloroplast during photosystem stoichiometry adjustment.在光系统化学计量调整过程中，叶绿体氧化还原信号对细胞核光合作用基因调控的一种新机制。
J Biol Chem. 2001 Sep 28;276(39):36125-30. doi: 10.1074/jbc.M105701200. Epub 2001 Jul 23.
8
Differential electron flow around photosystem I by two C(4)-photosynthetic-cell-specific ferredoxins.两种C4光合细胞特异性铁氧化还原蛋白在光系统I周围的差异电子流。
EMBO J. 2000 Oct 2;19(19):5041-50. doi: 10.1038/sj.emboj.7593319.
9
Electron flow to photosystem I from stromal reductants in vivo: the size of the pool of stromal reductants controls the rate of electron donation to both rapidly and slowly reducing photosystem I units.体内来自基质还原剂的电子流向光系统I：基质还原剂库的大小控制着向快速和慢速还原的光系统I单位的电子供体速率。
Planta. 2002 Sep;215(5):812-20. doi: 10.1007/s00425-002-0808-3. Epub 2002 Aug 8.
10
Analysis of donors of electrons to photosystem I and cyclic electron flow by redox kinetics of P700 in chloroplasts of isolated bundle sheath strands of maize.通过玉米离体维管束鞘细胞叶绿体中P700的氧化还原动力学分析光合系统I的电子供体和循环电子流。
Photosynth Res. 2007 Apr;92(1):65-74. doi: 10.1007/s11120-007-9166-0. Epub 2007 Jun 6.
引用本文的文献
1
Solid-like condensation of MORF8 inhibits RNA editing under heat stress in Arabidopsis.拟南芥中MORF8的固态样凝聚在热胁迫下抑制RNA编辑。
Nat Commun. 2025 Mar 21;16(1):2789. doi: 10.1038/s41467-025-58146-1.
2
Investigation of the effect of salt stress on photosynthetic electron transport pathways in the Synechocystis PCC 6803 cyanobacterium.盐胁迫对集胞藻PCC 6803蓝细菌光合电子传递途径影响的研究。
Physiol Plant. 2025 Jan-Feb;177(1):e70066. doi: 10.1111/ppl.70066.
3
Monitoring the photosynthetic activity at single-cell level in .监测……中单细胞水平的光合活性。
你提供的原文似乎不完整，“in”后面缺少具体内容。
Photosynthetica. 2023 Dec 18;61(4):473-482. doi: 10.32615/ps.2023.042. eCollection 2023.
4
Evaluation of visible-light wavelengths that reduce or oxidize the plastoquinone pool in green algae with the activated F rise method.采用激活的F上升法评估可降低或氧化绿藻中质体醌库的可见光波长。
Photosynthetica. 2022 Nov 24;60(4):529-538. doi: 10.32615/ps.2022.049. eCollection 2022.
5
Limiting steps and the contribution of alternative electron flow pathways in the recovery of the photosynthetic functions after freezing-induced desiccation of .冷冻诱导干燥后光合功能恢复过程中的限制步骤及替代电子流途径的贡献 。
需注意，你提供的原文最后“. ”表述不完整，可能影响对完整内容的理解。
Photosynthetica. 2022 Mar 7;60(1):136-146. doi: 10.32615/ps.2022.008. eCollection 2022.
6
RNA Polymerase RPOTp is Involved in C-to-U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts.RNA聚合酶RPOTp参与拟南芥叶绿体多个位点的C到U RNA编辑。
Adv Sci (Weinh). 2025 Jan;12(4):e2405131. doi: 10.1002/advs.202405131. Epub 2024 Dec 4.
7
Unlocking the adaptation mechanisms of the oleaginous microalga sp. BHU1 under elevated salt stress: a physiochemical, lipidomics and transcriptomics approach.揭示产油微藻sp. BHU1在盐胁迫升高条件下的适应机制：一种物理化学、脂质组学和转录组学方法
Front Microbiol. 2024 Nov 18;15:1475410. doi: 10.3389/fmicb.2024.1475410. eCollection 2024.
8
Photosynthetic Electron Flows and Networks of Metabolite Trafficking to Sustain Metabolism in Photosynthetic Systems.光合电子流与代谢物运输网络以维持光合系统中的新陈代谢
Plants (Basel). 2024 Oct 28;13(21):3015. doi: 10.3390/plants13213015.
9
Membrane protein provision controls prothylakoid biogenesis in tobacco etioplasts.膜蛋白供应控制烟草黄化质体中前质体类囊体的生物发生。
Plant Cell. 2024 Sep 25;36(12):4862-80. doi: 10.1093/plcell/koae259.
10
Lighting the way: Compelling open questions in photosynthesis research.照亮道路：光合作用研究中引人入胜的开放性问题。
Plant Cell. 2024 Oct 3;36(10):3914-3943. doi: 10.1093/plcell/koae203.
本文引用的文献
1
Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.新型调制荧光计连续记录光化学和非光化学叶绿素荧光猝灭。
Photosynth Res. 1986 Jan;10(1-2):51-62. doi: 10.1007/BF00024185.
2
The relationship between CO2 assimilation and electron transport in leaves.叶片中二氧化碳同化与电子传递之间的关系。
Photosynth Res. 1990 Sep;25(3):213-24. doi: 10.1007/BF00033162.
3
Regulation of photosynthetic electron transport and photophosphorylation in intact chloroplasts and leaves of Spinacia oleracea L.完整叶绿体和菠菜叶片中光合作用电子传递和光磷酸化的调节
Planta. 1978 Jan;143(1):41-9. doi: 10.1007/BF00389050.
4
Electron transport and photophosphorylation by Photosystem I in vivo in plants and cyanobacteria.植物和蓝藻中光系统 I 的电子传递和光合磷酸化。
Photosynth Res. 1993 Jun;36(3):149-68. doi: 10.1007/BF00033035.
5
Respiratory control over photosynthetic electron transport in chloroplasts of higher-plant cells: evidence for chlororespiration.高等植物细胞叶绿体中光合电子传递的呼吸控制：证据表明存在光呼吸。
Planta. 1989 Oct;179(3):349-58. doi: 10.1007/BF00391080.
6
Concerning a dual function of coupled cyclic electron transport in leaves.关于叶片中偶联循环电子传递的双重功能。
Plant Physiol. 1992 Dec;100(4):1621-6. doi: 10.1104/pp.100.4.1621.
7
Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.利用粒子轰击法稳定转化完整的烟草细胞。
Proc Natl Acad Sci U S A. 1988 Nov;85(22):8502-5. doi: 10.1073/pnas.85.22.8502.
8
Evidence for a respiratory chain in the chloroplast.叶绿体中呼吸链的证据。
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4352-6. doi: 10.1073/pnas.79.14.4352.
9
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.烟草叶绿体基因组的完整核苷酸序列：其基因组织与表达
EMBO J. 1986 Sep;5(9):2043-2049. doi: 10.1002/j.1460-2075.1986.tb04464.x.
10
REGULATION OF LIGHT HARVESTING IN GREEN PLANTS.绿色植物中光捕获的调控
Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:655-684. doi: 10.1146/annurev.arplant.47.1.655.
Zotero 插件