Villeda C J, Gómez-Villamandos J C, Williams S M, Hervás J, Wilkinson P J, Viñuela E
Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Thromb Haemost. 1995 Jan;73(1):112-7.
The activity of several proteins involved in fibrinolysis and the morphological changes in the blood vessel walls of pigs infected with highly virulent (Malawi'83) and moderately virulent (Dominican Republic '78-DR'78) ASF virus isolates were determined. Pigs infected with the Malawi'83 virus developed an increased fibrinolytic activity due to high plasma levels of tissue-plasminogen activator (t-PA) of 71.3 +/- 22.8 IU/ml (mean +/- SD), which correlated well with an increased activation of interstitial capillary endothelial cells and high levels of 1150 +/- 73.6 nM of fibrin monomer in the circulation. Animals infected with DR'78 virus, in contrast, showed an inhibition of fibrinolysis in the late stages of disease with almost a 5-fold increase of plasminogen activator inhibitor (PAI) activity of 196.0 AU/ml. These results suggest that activation of the fibrinolytic system in pigs infected with the Malawi'83 virus is probably due to increased formation and deposition of fibrin in the circulation, contributing to an increased bleeding tendency and higher mortality. On the contrary, animals infected with DR'78 virus developed an inhibition of fibrinolysis and thus a reduction in bleeding.
测定了感染高致病性（马拉维'83）和中等致病性（多米尼加共和国'78 - DR'78）非洲猪瘟病毒分离株的猪体内几种参与纤维蛋白溶解的蛋白质的活性以及血管壁的形态变化。感染马拉维'83病毒的猪由于血浆中组织型纤溶酶原激活剂（t - PA）水平较高，达到71.3±22.8 IU/ml（平均值±标准差），其纤维蛋白溶解活性增强，这与间质毛细血管内皮细胞的激活增加以及循环中1150±73.6 nM的纤维蛋白单体水平升高密切相关。相比之下，感染DR'78病毒的动物在疾病后期表现出纤维蛋白溶解受到抑制，纤溶酶原激活剂抑制剂（PAI）活性几乎增加了5倍，达到196.0 AU/ml。这些结果表明，感染马拉维'83病毒的猪体内纤维蛋白溶解系统的激活可能是由于循环中纤维蛋白的形成和沉积增加，导致出血倾向增加和死亡率升高。相反，感染DR'78病毒的动物出现纤维蛋白溶解受到抑制，从而出血减少。
