带帽mRNA降解中间体在酵母spb8-2突变体中积累。
Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.
作者信息
Boeck R, Lapeyre B, Brown C E, Sachs A B
机构信息
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
出版信息
Mol Cell Biol. 1998 Sep;18(9):5062-72. doi: 10.1128/MCB.18.9.5062.
Abstract
mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3' end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3'-5' mRNA degradation intermediates in yeast.
摘要
酿酒酵母中的mRNA主要通过一种由mRNA帽结构去除所刺激的途径进行降解。在此我们报告，最初被鉴定为聚腺苷酸结合蛋白（PAB1）基因缺失的抑制突变的SPB8（YJL124c）基因中的一个突变，可稳定mRNA帽结构。具体而言，我们发现spb8 - 2突变导致带帽、缺乏聚腺苷酸的mRNA积累。该突变的存在还使得能够检测从3'端修剪的mRNA种类。这些数据表明，这个类Sm蛋白家族成员参与了mRNA去帽过程，并且它们提供了酵母中3' - 5'mRNA降解中间体的一个例子。
相似文献
1
Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.带帽mRNA降解中间体在酵母spb8-2突变体中积累。
Mol Cell Biol. 1998 Sep;18(9):5062-72. doi: 10.1128/MCB.18.9.5062.
2
Decapping of stabilized, polyadenylated mRNA in yeast pab1 mutants.酵母pab1突变体中稳定的多聚腺苷酸化mRNA的去帽作用。
Yeast. 1999 Jun 15;15(8):687-702. doi: 10.1002/(SICI)1097-0061(19990615)15:8<687::AID-YEA412>3.0.CO;2-L.
3
Two related proteins, Edc1p and Edc2p, stimulate mRNA decapping in Saccharomyces cerevisiae.两种相关蛋白Edc1p和Edc2p可刺激酿酒酵母中的mRNA脱帽反应。
Genetics. 2001 Jan;157(1):27-37. doi: 10.1093/genetics/157.1.27.
4
mRNA decapping activities and their biological roles.信使核糖核酸脱帽活性及其生物学作用。
Biochimie. 1996;78(11-12):1049-55. doi: 10.1016/s0300-9084(97)86729-6.
5
An essential component of the decapping enzyme required for normal rates of mRNA turnover.正常mRNA周转率所需的脱帽酶的一个重要组成部分。
Nature. 1996 Aug 15;382(6592):642-6. doi: 10.1038/382642a0.
6
Mechanisms and control of mRNA decapping in Saccharomyces cerevisiae.酿酒酵母中mRNA脱帽的机制与调控
Annu Rev Biochem. 2000;69:571-95. doi: 10.1146/annurev.biochem.69.1.571.
7
The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p.两种蛋白质Pat1p（Mrt1p）和Spb8p在体内相互作用，是mRNA降解所必需的，并且在功能上与Pab1p相关联。
Mol Cell Biol. 2000 Aug;20(16):5939-46. doi: 10.1128/MCB.20.16.5939-5946.2000.
8
Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system.双链RNA病毒对帽状mRNA降解系统的诱骗以及酵母抗病毒系统对聚腺苷酸化mRNA的监测。
Mol Cell Biol. 1995 May;15(5):2763-71. doi: 10.1128/MCB.15.5.2763.
9
Yeast Sm-like proteins function in mRNA decapping and decay.酵母Sm样蛋白在mRNA脱帽和降解过程中发挥作用。
Nature. 2000 Mar 30;404(6777):515-8. doi: 10.1038/35006676.
10
Targeting an mRNA for decapping: displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs.靶向用于脱帽的mRNA：翻译因子的置换以及Lsm1p - 7p复合物与去腺苷酸化酵母mRNA的结合
Mol Cell. 2001 Nov;8(5):1075-83. doi: 10.1016/s1097-2765(01)00395-1.
引用本文的文献
1
Quantitative reconstitution of yeast RNA processing bodies.酵母 RNA 处理体的定量重构。
Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 10.1073/pnas.2214064120. Epub 2023 Mar 27.
2
Eukaryotic mRNA decapping factors: molecular mechanisms and activity.真核生物 mRNA 脱帽因子：分子机制与活性。
FEBS J. 2023 Nov;290(21):5057-5085. doi: 10.1111/febs.16626. Epub 2022 Sep 30.
3
Eukaryotic mRNA Decapping Activation.真核生物mRNA脱帽激活
Front Genet. 2022 Mar 23;13:832547. doi: 10.3389/fgene.2022.832547. eCollection 2022.
4
PABP prevents the untimely decay of select mRNA populations in human cells.PABP 防止了人类细胞中特定 mRNA 群体的过早降解。
EMBO J. 2022 Mar 15;41(6):e108650. doi: 10.15252/embj.2021108650. Epub 2022 Feb 14.
5
Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function.酵母 RNA 外切体亚基 Rrp44 的核仁定位暗示其主要功能是早期前 rRNA 加工。
J Biol Chem. 2020 Aug 7;295(32):11195-11213. doi: 10.1074/jbc.RA120.013589. Epub 2020 Jun 17.
6
Emerging Roles of LSM Complexes in Posttranscriptional Regulation of Plant Response to Abiotic Stress.LSM复合物在植物对非生物胁迫响应的转录后调控中的新作用
Front Plant Sci. 2019 Feb 19;10:167. doi: 10.3389/fpls.2019.00167. eCollection 2019.
7
Multispecies reconstructions uncover widespread conservation, and lineage-specific elaborations in eukaryotic mRNA metabolism.多物种重建揭示了真核生物 mRNA 代谢中的广泛保守性和谱系特异性的精细化。
PLoS One. 2018 Mar 21;13(3):e0192633. doi: 10.1371/journal.pone.0192633. eCollection 2018.
8
P-body proteins regulate transcriptional rewiring to promote DNA replication stress resistance.P小体蛋白调节转录重排以促进DNA复制应激抗性。
Nat Commun. 2017 Sep 15;8(1):558. doi: 10.1038/s41467-017-00632-2.
9
The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes.ApaH样磷酸酶TbALPH1是锥虫的主要mRNA去帽酶。
PLoS Pathog. 2017 Jun 19;13(6):e1006456. doi: 10.1371/journal.ppat.1006456. eCollection 2017 Jun.
10
Mutagenic Analysis of the C-Terminal Extension of Lsm1.Lsm1 C 末端延伸区的诱变分析
PLoS One. 2016 Jul 19;11(7):e0158876. doi: 10.1371/journal.pone.0158876. eCollection 2016.
本文引用的文献
1
The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.酵母mRNA的3'至5'降解是mRNA周转的一种普遍机制，该机制需要SKI2 DEVH盒蛋白和外切体复合物的3'至5'核酸外切酶。
EMBO J. 1998 Mar 2;17(5):1497-506. doi: 10.1093/emboj/17.5.1497.
2
RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G.酵母Pab1p的RNA识别基序2是其与真核生物翻译起始因子4G进行功能相互作用所必需的。
Mol Cell Biol. 1998 Jan;18(1):51-7. doi: 10.1128/MCB.18.1.51.
3
The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.外泌体：一种保守的真核生物RNA加工复合体，含有多种3'→5'外切核糖核酸酶。
Cell. 1997 Nov 14;91(4):457-66. doi: 10.1016/s0092-8674(00)80432-8.
4
Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation.翻译起始因子eIF4G介导体外多聚腺苷酸尾依赖性翻译。
Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9046-51. doi: 10.1073/pnas.94.17.9046.
5
CaSm: an Sm-like protein that contributes to the transformed state in cancer cells.CaSm：一种类似Sm的蛋白质，它与癌细胞的转化状态有关。
Cancer Res. 1997 Jul 15;57(14):2961-5.
6
Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens.通过全面的双杂交筛选对酵母基因组进行功能分析。
Nat Genet. 1997 Jul;16(3):277-82. doi: 10.1038/ng0797-277.
7
Starting at the beginning, middle, and end: translation initiation in eukaryotes.从起始、中间和结尾开始：真核生物中的翻译起始
Cell. 1997 Jun 13;89(6):831-8. doi: 10.1016/s0092-8674(00)80268-8.
8
Life and death in the cytoplasm: messages from the 3' end.细胞质中的生死：来自3'端的信息
Curr Opin Genet Dev. 1997 Apr;7(2):220-32. doi: 10.1016/s0959-437x(97)80132-3.
9
Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G.酵母聚腺苷酸尾结合蛋白与翻译起始因子eIF-4G的关联。
EMBO J. 1996 Dec 16;15(24):7168-77.
10
Making sense of nonsense in yeast.解读酵母中的无意义现象。
Trends Biochem Sci. 1996 Nov;21(11):433-8. doi: 10.1016/s0968-0004(96)10055-4.
Zotero 插件