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The CUP1 upstream repeated element renders CUP1 promoter activation insensitive to mutations in the RNA polymerase II transcription complex.CUP1上游重复元件使CUP1启动子激活对RNA聚合酶II转录复合物中的突变不敏感。
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Transcriptional activation independent of TFIIH kinase and the RNA polymerase II mediator in vivo.体内不依赖TFIIH激酶和RNA聚合酶II中介体的转录激活
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本文引用的文献

1
Transcriptional activation independent of TFIIH kinase and the RNA polymerase II mediator in vivo.体内不依赖TFIIH激酶和RNA聚合酶II中介体的转录激活
Nature. 1998 May 28;393(6683):389-92. doi: 10.1038/30770.
2
RNA polymerase I-promoted HIS4 expression yields uncapped, polyadenylated mRNA that is unstable and inefficiently translated in Saccharomyces cerevisiae.RNA聚合酶I促进的HIS4表达产生无帽、多聚腺苷酸化的mRNA，其在酿酒酵母中不稳定且翻译效率低下。
Mol Cell Biol. 1998 Feb;18(2):665-75. doi: 10.1128/MCB.18.2.665.
3
Genetics of transcriptional regulation in yeast: connections to the RNA polymerase II CTD.酵母转录调控的遗传学：与RNA聚合酶II CTD的联系
Annu Rev Cell Dev Biol. 1997;13:1-23. doi: 10.1146/annurev.cellbio.13.1.1.
4
The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.酵母的中介蛋白及其通过RNA聚合酶II羧基末端结构域的功能。
Genes Dev. 1998 Jan 1;12(1):45-54. doi: 10.1101/gad.12.1.45.
5
mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain.mRNA加帽酶通过RNA聚合酶II羧基末端结构域的磷酸化被招募到转录复合物中。
Genes Dev. 1997 Dec 15;11(24):3319-26. doi: 10.1101/gad.11.24.3319.
6
5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II.5'-加帽酶通过与RNA聚合酶II的磷酸化羧基末端结构域结合，被靶向到前体mRNA上。
Genes Dev. 1997 Dec 15;11(24):3306-18. doi: 10.1101/gad.11.24.3306.
7
Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators.延伸因子TFIIS和延伸素A与一种能够进行启动子特异性起始并对转录激活因子作出反应的人RNA聚合酶II全酶的相互作用。
J Biol Chem. 1997 Sep 26;272(39):24563-71. doi: 10.1074/jbc.272.39.24563.
8
Yeast TAF(II)145 functions as a core promoter selectivity factor, not a general coactivator.酵母TAF(II)145作为核心启动子选择性因子，而非一般的共激活因子发挥作用。
Cell. 1997 Aug 22;90(4):615-24. doi: 10.1016/s0092-8674(00)80523-1.
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Evidence for a mediator cycle at the initiation of transcription.转录起始时介质循环的证据。
Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6075-8. doi: 10.1073/pnas.94.12.6075.
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Transcriptional activation by recruitment.通过募集实现转录激活
Nature. 1997 Apr 10;386(6625):569-77. doi: 10.1038/386569a0.

芽殖酵母中不依赖RNA聚合酶II全酶的激活转录

Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast.

作者信息

McNeil J B, Agah H, Bentley D

机构信息

Amgen Institute, Ontario Cancer Institute, Toronto, Ontario M5G 2C1, Canada.

出版信息

Genes Dev. 1998 Aug 15;12(16):2510-21. doi: 10.1101/gad.12.16.2510.

DOI:10.1101/gad.12.16.2510
PMID:9716404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC317099/
Abstract

We investigated whether the multisubunit holoenzyme complex of RNA polymerase II (Pol II) and mediator is universally required for transcription in budding yeast. DeltaCTD Pol II lacking the carboxy-terminal domain of the large subunit cannot assemble with mediator but can still transcribe the CUP1 gene. CUP1 transcripts made by DeltaCTD Pol II initiated correctly and some extended past the normal poly(A) site yielding a novel dicistronic mRNA. Most CUP1 transcripts made by DeltaCTD Pol II were degraded but could be stabilized by deletion of the XRN1 gene. Unlike other genes, transcription of CUP1 and HSP82 also persisted after inactivation of the CTD kinase Kin28 or the mediator subunit Srb4. The upstream-activating sequence (UAS) of the CUP1 promoter was sufficient to drive Cu2+ inducible transcription without Srb4 and heat shock inducible transcription without the CTD. We conclude that the Pol II holoenzyme is not essential for all UAS-dependent activated transcription in yeast.

摘要

我们研究了RNA聚合酶II（Pol II）和中介体的多亚基全酶复合物对于芽殖酵母转录是否普遍必需。缺乏大亚基羧基末端结构域的DeltaCTD Pol II不能与中介体组装，但仍能转录CUP1基因。由DeltaCTD Pol II产生的CUP1转录本起始正确，一些转录本延伸超过正常的聚腺苷酸化位点，产生一种新的双顺反子mRNA。由DeltaCTD Pol II产生的大多数CUP1转录本被降解，但通过缺失XRN1基因可使其稳定。与其他基因不同，在CTD激酶Kin28或中介体亚基Srb4失活后，CUP1和HSP82的转录仍持续。CUP1启动子的上游激活序列（UAS）足以驱动Cu2+诱导的转录（无需Srb4）以及热休克诱导的转录（无需CTD）。我们得出结论，Pol II全酶对于酵母中所有依赖UAS的激活转录并非必不可少。