芽殖酵母中不依赖RNA聚合酶II全酶的激活转录
Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast.
作者信息
McNeil J B, Agah H, Bentley D
机构信息
Amgen Institute, Ontario Cancer Institute, Toronto, Ontario M5G 2C1, Canada.
出版信息
Genes Dev. 1998 Aug 15;12(16):2510-21. doi: 10.1101/gad.12.16.2510.
Abstract
We investigated whether the multisubunit holoenzyme complex of RNA polymerase II (Pol II) and mediator is universally required for transcription in budding yeast. DeltaCTD Pol II lacking the carboxy-terminal domain of the large subunit cannot assemble with mediator but can still transcribe the CUP1 gene. CUP1 transcripts made by DeltaCTD Pol II initiated correctly and some extended past the normal poly(A) site yielding a novel dicistronic mRNA. Most CUP1 transcripts made by DeltaCTD Pol II were degraded but could be stabilized by deletion of the XRN1 gene. Unlike other genes, transcription of CUP1 and HSP82 also persisted after inactivation of the CTD kinase Kin28 or the mediator subunit Srb4. The upstream-activating sequence (UAS) of the CUP1 promoter was sufficient to drive Cu2+ inducible transcription without Srb4 and heat shock inducible transcription without the CTD. We conclude that the Pol II holoenzyme is not essential for all UAS-dependent activated transcription in yeast.
摘要
我们研究了RNA聚合酶II（Pol II）和中介体的多亚基全酶复合物对于芽殖酵母转录是否普遍必需。缺乏大亚基羧基末端结构域的DeltaCTD Pol II不能与中介体组装，但仍能转录CUP1基因。由DeltaCTD Pol II产生的CUP1转录本起始正确，一些转录本延伸超过正常的聚腺苷酸化位点，产生一种新的双顺反子mRNA。由DeltaCTD Pol II产生的大多数CUP1转录本被降解，但通过缺失XRN1基因可使其稳定。与其他基因不同，在CTD激酶Kin28或中介体亚基Srb4失活后，CUP1和HSP82的转录仍持续。CUP1启动子的上游激活序列（UAS）足以驱动Cu2+诱导的转录（无需Srb4）以及热休克诱导的转录（无需CTD）。我们得出结论，Pol II全酶对于酵母中所有依赖UAS的激活转录并非必不可少。
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本文引用的文献
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RNA polymerase I-promoted HIS4 expression yields uncapped, polyadenylated mRNA that is unstable and inefficiently translated in Saccharomyces cerevisiae.RNA聚合酶I促进的HIS4表达产生无帽、多聚腺苷酸化的mRNA，其在酿酒酵母中不稳定且翻译效率低下。
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