Hsieh L L, Lin P J, Chen T C, Ou J T
Department of Public Health, Chang Gung College of Medicine and Technology, Taiwan, PR China.
Cancer Lett. 1998 Jul 17;129(2):125-9. doi: 10.1016/s0304-3835(98)00111-6.
Eighty-two gastric adenocarcinomas (32 intestinal and 50 diffuse type) were investigated for the presence of Epstein-Barr virus (EBV) DNA by amplifying the 78-bp fragment from the EBNA1 gene with polymerase chain reaction. EBV was detected in 17 (20.7%) of the 82 gastric tumorous specimens. Of these 17 EBV-positive cases, only one was EBV-positive in the adjacent non-tumorous tissue. EBV-positive gastric adenocarcinoma was present in 15.6 and 24.0% of the intestinal and diffuse type tumors, respectively, but within the two classes there was significant non-homogeneity by type. EBV was found more frequently in adenocarcinomas of the tubular (25%) and poorly differentiated (30.3%) types. EBV involvement was found more in male than in female patients. Eighty of the 82 tumors were also checked for the prevalence of p53 gene mutation. Of the 17 EBV-positive gastric adenocarcinomas, only two showed p53 gene mutations. The p53 mutation rate was lower in EBV-positive tumors (11.8%) than in EBV-negative tumors (25.4%).
通过聚合酶链反应扩增EBNA1基因的78碱基对片段，对82例胃腺癌（32例肠型和50例弥漫型）进行了爱泼斯坦-巴尔病毒（EBV）DNA检测。在82例胃肿瘤标本中，17例（20.7%）检测到EBV。在这17例EBV阳性病例中，仅1例在相邻的非肿瘤组织中呈EBV阳性。EBV阳性的胃腺癌在肠型和弥漫型肿瘤中分别占15.6%和24.0%，但在这两类肿瘤中，按类型存在显著的非均一性。在管状腺癌（25%）和低分化腺癌（30.3%）中EBV的检出更为频繁。EBV感染在男性患者中比在女性患者中更为常见。82例肿瘤中的80例还检测了p53基因突变的发生率。在17例EBV阳性的胃腺癌中，仅2例显示p53基因突变。EBV阳性肿瘤的p53突变率（11.8%）低于EBV阴性肿瘤（25.4%）。
