生长环境对兔多形核白细胞和阳离子蛋白杀灭铜绿假单胞菌的影响。
Effect of growth environment on Pseudomonas aeruginosa killing by rabbit polymorphonuclear leudocytes and cationic proteins.
作者信息
Finch J E, Brown M R
出版信息
Infect Immun. 1978 May;20(2):340-6. doi: 10.1128/iai.20.2.340-346.1978.
Pseudomonas aeruginosa grown in a chemostat under carbon- and magnesium-limited conditions showed varying resistance to killing by rabbit peritoneal exudate polymorphonuclear leukocytes. Slow-growing (D = 0.05 h-1), magnesium-limited cells were significantly more resistant to the lethal effects of the phagocytes than were fast-growing magnesium-limited cells and carbon-limited cells (D = 0.05 h-1 and D = 0.5 h-1, respectively). The resistance of magnesium-limited cells to killing by cationic proteins isolated from the leukocytes was shown to be growth-rate dependent, the slowest-growing (D = 0.05 h-1) cells being the most resistant. Carbon-limited cells were sensitive to killing by the cationic proteins at all growth rates tested. Antisera raised in rabbits to all types of cells and commercial anti-Pseudomonas serum rapidly agglutinated magnesium-limited cells but failed to agglutinate carbon-limited cells. There was some indication that slow-growing (D = 0.05 h-1), magnesium-limited cells agglutinated most readily with both types of antisera. No difference was detected in the mouse toxicity of heat-killed cells grown under the various conditions.
在恒化器中于碳和镁限制条件下培养的铜绿假单胞菌对兔腹膜渗出多形核白细胞的杀伤表现出不同的抗性。生长缓慢（D = 0.05 h⁻¹）、镁限制的细胞比快速生长的镁限制细胞和碳限制细胞（分别为D = 0.05 h⁻¹和D = 0.5 h⁻¹）对吞噬细胞的致死作用具有显著更高的抗性。镁限制细胞对从白细胞中分离的阳离子蛋白杀伤的抗性显示出与生长速率相关，生长最慢（D = 0.05 h⁻¹）的细胞抗性最强。在所有测试的生长速率下，碳限制细胞对阳离子蛋白的杀伤敏感。用兔制备的针对所有类型细胞的抗血清和商业抗假单胞菌血清能迅速凝集镁限制细胞，但不能凝集碳限制细胞。有迹象表明，生长缓慢（D = 0.05 h⁻¹）的镁限制细胞与两种抗血清的凝集最容易。在不同条件下生长的热灭活细胞的小鼠毒性未检测到差异。
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