半乳甘露聚糖曲霉的检测：酶联免疫测定法与铕标记时间分辨荧光免疫测定法的比较
Detection of Aspergillus galactomannan: comparison of an enzyme-linked immunoassay and a europium-linked time-resolved fluoroimmunoassay.
作者信息
Paugam A, Sarfati J, Romieu R, Viguier M, Dupouy-Camet J, Latgé J P
机构信息
Laboratoire de Mycologie, Hôpital Cochin, Paris, France.
出版信息
J Clin Microbiol. 1998 Oct;36(10):3079-80. doi: 10.1128/JCM.36.10.3079-3080.1998.
Abstract
With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an enzyme-linked immunosorbent assay using a peroxidase-conjugated detector antibody. No increase in the sensitivity of the detection of GM standards was seen with the europium-based fluoroimmunoassay.
摘要
为提高半乳甘露聚糖（GM）血清学检测的灵敏度，开发了一种铕标记时间分辨荧光免疫分析法。该方法与使用过氧化物酶标记检测抗体的酶联免疫吸附测定法进行了比较。基于铕的荧光免疫分析法在检测GM标准品时未发现灵敏度提高。
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