Ciantar M, Caruana D J
Department of Dental Surgery, University of Malta, Msida, Malta.
J Periodontal Res. 1998 Jul;33(5):259-64. doi: 10.1111/j.1600-0765.1998.tb02198.x.
The Periotron is an instrument designed to quantify submicrolitre volumes of fluid sampled on a filter paper strip. To date 3 models have been manufactured: the Periotron 600 (1976), the Periotron 6000 (1983) and more recently the Periotron 8000 (1995). This paper investigated for the first time the calibration characteristics and reliability of the Periotron 8000. The fluids under investigation were: de-ionised water, human serum, fetal bovine serum and an ultrafiltrate of fetal bovine serum. Quantitative analysis was studied by recording a series of Periotron readings over a volume range of 0-1.0 microliters for each fluid. The average of 5 Periotron values for each particular fluid was then plotted versus the respective fluid volume. Qualitative changes in fluid composition versus Periotron Scores were also analysed. Volume conversion for Periotron scores using both Periotron MLCONVRT software and a best fit equation selected from TableCurve 2D software compared well. The results of this study revealed that: 1) differences in calibration fluid composition (e.g. protein content) are reflected in the Periotron scores; 2) positioning of filter paper strip between the jaws of the Periotron should be standardised, 3) calibration of the Periotron 8000 seems to be consistent over a 1-wk interval.
牙周测量仪是一种用于对在滤纸条上采集的亚微升体积的液体进行定量的仪器。迄今为止，已生产了3种型号：牙周测量仪600（1976年）、牙周测量仪6000（1983年），以及最近的牙周测量仪8000（1995年）。本文首次研究了牙周测量仪8000的校准特性和可靠性。所研究的液体包括：去离子水、人血清、胎牛血清以及胎牛血清的超滤液。通过记录每种液体在0 - 1.0微升体积范围内的一系列牙周测量仪读数来研究定量分析。然后将每种特定液体的5个牙周测量仪值的平均值与相应的液体体积作图。还分析了液体成分相对于牙周测量仪分数的定性变化。使用牙周测量仪MLCONVRT软件以及从TableCurve 2D软件中选择的最佳拟合方程对牙周测量仪分数进行体积转换，结果吻合良好。本研究结果表明：1）校准液体成分（如蛋白质含量）的差异反映在牙周测量仪分数中；2）滤纸条在牙周测量仪夹口之间的放置应标准化；3）牙周测量仪8000在1周的时间间隔内校准似乎是一致的。
