模拟半程马拉松赛后血清抗氧化能力升高及血浆丙二醛浓度变化

Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to a simulated half-marathon run.

作者信息

Child R B, Wilkinson D M, Fallowfield J L, Donnelly A E

机构信息

Muscle Research Centre, Department of Medicine, Liverpool University, United Kingdom.

出版信息

Med Sci Sports Exerc. 1998 Nov;30(11):1603-7. doi: 10.1097/00005768-199811000-00008.

DOI:10.1097/00005768-199811000-00008

PMID:9813873

Abstract

PURPOSE AND METHODS

Indices of antioxidant status, membrane permeability, and lipid peroxidation were investigated in venous blood immediately before and after a simulated half-marathon run. In serum, these included the ability to scavenge free radicals (total antioxidant capacity, TAC), the concentration of uric acid (UA), and the activities of creatine kinase (CK) and beta-glucuronidase (beta G). The plasma concentration of malondialdehyde (MDA) was used as a marker of lipid peroxidation. Data were analyzed with paired t-tests. After a standardized warm-up, 17 trained male runners (mean +/- SD, age 31 +/- 4 yr, peak VO2 63.2 +/- 4.8 mL.kg-1.min-1) each completed a self-paced half-marathon run, on a motorized treadmill. Average exercise intensity was 77.1 +/- 1.0% peak VO2, with a performance time of 87.1 +/- 7.0 min.

RESULTS

After exercise, elevations were observed in MDA from 1.48 +/- 0.39 mmol.L-1 to 1.65 +/- 0.32 mmol.L-1 (P < 0.05), TAC from 475 +/- 84 to 564 +/- 113 mmol Trolox Eq.L-1 (P < 0.0001), UA from 268 +/- 45 to 312 +/- 51 mmol.L-1 (P < 0.001), serum cortisol concentration from 339 +/- 95 to 557 +/- 157 nmol.L-1 (P < 0.01), CK from 98 +/- 67 to 133 +/- 89 IU.L-1 (P < 0.0001), and beta G from 15.39 +/- 5.34 to 17.05 +/- 5.7 Sigma Units.mL-1 (P < 0.001).

CONCLUSIONS

The rise in TAC did not prevent exercise-induced lipid peroxidation and muscle damage as both MDA and CK were elevated after exercise. This may indicate inadequacies in the antioxidant defense system during the half-marathon run.

摘要

目的与方法

在模拟半程马拉松跑步前后即刻，对静脉血中的抗氧化状态、膜通透性和脂质过氧化指标进行了研究。在血清中，这些指标包括清除自由基的能力（总抗氧化能力，TAC）、尿酸（UA）浓度、肌酸激酶（CK）和β-葡萄糖醛酸酶（βG）的活性。丙二醛（MDA）的血浆浓度用作脂质过氧化的标志物。数据采用配对t检验进行分析。在进行标准化热身之后，17名训练有素的男性跑步者（平均±标准差，年龄31±4岁，峰值摄氧量63.2±4.8 mL·kg⁻¹·min⁻¹）在电动跑步机上各自完成了一次自定节奏的半程马拉松跑步。平均运动强度为峰值摄氧量的77.1±1.0%，成绩时间为87.1±7.0分钟。

结果

运动后，MDA从1.48±0.39 mmol·L⁻¹升高至1.65±0.32 mmol·L⁻¹（P<0.05），TAC从475±84升高至564±113 mmol Trolox Eq·L⁻¹（P<0.0001），UA从268±45升高至312±51 mmol·L⁻¹（P<0.001），血清皮质醇浓度从339±95升高至557±157 nmol·L⁻¹（P<0.01），CK从98±67升高至133±89 IU·L⁻¹（P<0.0001），βG从15.39±5.34升高至17.05±5.7西格玛单位·mL⁻¹（P<0.001）。