Stefanski V, Engler H
Department of Animal Physiology, University of Bayreuth, Germany.
Physiol Behav. 1998 Jul;64(5):733-41. doi: 10.1016/s0031-9384(98)00127-9.
The study compares the impact of acute and chronic social confrontation on aspects of blood cellular immunity in Long-Evans intruder rats. An adult male was introduced for either 2 h or 48 h into a male-female resident group, which resulted in fights for dominance. Thirty-eight of the 42 intruders became losers. For immunologic measurements, blood samples were taken from the intruders before confrontation (baseline) and 2 h or 48 h after the beginning of confrontation. Two h of confrontation resulted in increased granulocyte (+65%) and decreased lymphocyte numbers (-60%), as well as in differential reductions in CD4, CD8, and B cell numbers. CD4/CD8 and T/B ratios were elevated. T cell responsiveness to ConA was markedly suppressed in proliferation assays using either whole blood (-90%) or PBMC (-50%). The direction of changes in leukocyte and lymphocyte subsets after 48 h resembled in many aspects the 2 h changes, although with lower magnitude. In contrast to acute stress, a lowered T/B cells ratio and unaffected CD4/CD8 ratio was determined after 48 h. Proliferative response of T cells was lowered by about 25% in the whole blood assay; but unaffected in the PBMC assay. Significant correlations were found between the amount of submissive behavior displayed by the losers and several immunologic measures after 2 h of confrontation. The data suggest that acute and chronic stressful conditions may not necessarily result in similar effects on immune functioning. This should be considered when evaluating the biologic and evolutionary consequences of social stress-induced immune alterations.
该研究比较了急性和慢性社会对抗对Long-Evans侵入大鼠血细胞免疫方面的影响。将一只成年雄性大鼠放入一个雌雄混居的群体中2小时或48小时，这导致了争夺主导权的争斗。42只侵入大鼠中有38只成为失败者。为了进行免疫学测量，在对抗前（基线）以及对抗开始后2小时或48小时从侵入大鼠身上采集血样。2小时的对抗导致粒细胞数量增加（+65%）、淋巴细胞数量减少（-60%），以及CD4、CD8和B细胞数量的差异性减少。CD4/CD8和T/B比率升高。在使用全血（-90%）或外周血单核细胞（PBMC，-50%）的增殖试验中，T细胞对刀豆蛋白A的反应性明显受到抑制。48小时后白细胞和淋巴细胞亚群的变化方向在许多方面与2小时的变化相似，尽管幅度较小。与急性应激相反，48小时后测定T/B细胞比率降低而CD4/CD8比率未受影响。在全血试验中T细胞的增殖反应降低了约25%；但在PBMC试验中未受影响。在对抗2小时后，失败者表现出的顺从行为量与几种免疫学指标之间发现了显著相关性。数据表明，急性和慢性应激条件对免疫功能的影响不一定相似。在评估社会应激诱导的免疫改变的生物学和进化后果时应考虑到这一点。
