Saeed M F, Nunes M, Vasconcelos P F, Travassos Da Rosa A P, Watts D M, Russell K, Shope R E, Tesh R B, Barrett A D
Departments of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USA. .
J Clin Microbiol. 2001 Jul;39(7):2445-52. doi: 10.1128/JCM.39.7.2445-2452.2001.
Oropouche (ORO) virus is an emerging infectious agent that has caused numerous outbreaks of an acute febrile (dengue-like) illness among humans in Brazil, Peru, and Panama. Diagnosis of ORO virus infection is based mainly on serology. Two different antigens, hamster serum antigen (HSA) and Vero cell lysate antigen (VCLA), are currently used in enzyme immunoassays (EIAs) in Brazil and Peru, respectively, to investigate the epidemiology of ORO virus infection. Both antigens involve use of infectious virus, and for this reason their use is restricted. Consequently, the frequency and distribution of ORO virus infection are largely unexplored in other countries of South America. This report describes the use of a bacterially expressed recombinant nucleocapsid (rN) protein of ORO virus in EIAs for the diagnosis of ORO virus infection. The data revealed that the purified rN protein is comparable to the authentic viral N protein in its antigenic characteristics and is highly sensitive and specific in EIAs. Among 183 serum samples tested, a high degree of concordance was found between rN protein-based EIA and HSA- and VCLA-based EIAs for the detection of both ORO virus-specific immunoglobulin M (IgM) and IgG antibodies. The high sensitivity, specificity, and safety of the rN protein-based EIA make it a useful diagnostic technique that can be widely used to detect ORO virus infection in South America.
奥罗普切（ORO）病毒是一种新出现的传染病原体，在巴西、秘鲁和巴拿马曾引发多起人类急性发热（类似登革热）疾病疫情。ORO病毒感染的诊断主要基于血清学检测。目前在巴西和秘鲁，酶免疫测定（EIA）分别使用两种不同的抗原，即仓鼠血清抗原（HSA）和非洲绿猴肾细胞裂解物抗原（VCLA）来调查ORO病毒感染的流行病学情况。这两种抗原都涉及传染性病毒的使用，因此其应用受到限制。所以，南美洲其他国家ORO病毒感染的频率和分布情况在很大程度上尚未得到探究。本报告描述了将ORO病毒的细菌表达重组核衣壳（rN）蛋白用于EIA以诊断ORO病毒感染的情况。数据显示，纯化的rN蛋白在抗原特性方面与天然病毒N蛋白相当，并且在EIA中具有高度敏感性和特异性。在检测的183份血清样本中，基于rN蛋白的EIA与基于HSA和VCLA的EIA在检测ORO病毒特异性免疫球蛋白M（IgM）和IgG抗体方面具有高度一致性。基于rN蛋白的EIA具有高敏感性、特异性和安全性，使其成为一种有用的诊断技术，可广泛用于检测南美洲的ORO病毒感染。
