Kardinaal A F, Morton M S, Brüggemann-Rotgans I E, van Beresteijn E C
Department of Consumer Research and Epidemiology, TNO Nutrition and Food Research Institute, Zeist, The Netherlands.
Eur J Clin Nutr. 1998 Nov;52(11):850-5. doi: 10.1038/sj.ejcn.1600659.
The hypothesis was tested that the rate of postmenopausal bone loss is inversely associated with long-term urinary excretion of phyto-oestrogens, as a marker of habitual dietary intake.
Secondary analysis of a 10-year follow-up study (1979 1989) among postmenopausal women in the Netherlands.
From the original population of 154 women, 32 women were selected with an annual rate of radial bone loss of < or = 0.5% over the first 5 years of the study and 35 women with a rate of > or = 2.5% per year.
The isoflavonoids genistein, daidzein and equol, and the lignan enterolactone were determined by gas chromatography mass spectrometry in aggregate samples from annually collected urine samples. Cortical bone density of the radius had previously been measured annually by single-photon absorptiometry.
Excretion of isoflavonoids did not differ between both groups, although in multivariate analysis equol excretion was weakly positively associated with rate of bone loss in the 5 years after the menopause. Enterolactone excretion was significantly higher in the group with high rate of bone loss. This positive association remained in multivariate linear regression analysis after adjustment for age, years since menopause, body mass index and intake of calcium, vegetable protein and dietary fibre.
Enterolactone excretion is likely to be an indicator of consumption of grains and legumes; it is not clear whether the observed positive association with rate of bone loss is a causal one. Our results do not support a preventive effect of low, unsupplemented dietary intake of phyto-oestrogens on postmenopausal cortical bone loss. However, no conclusions can be drawn about effects of higher doses of phyto-oestrogens.
检验这样一个假设，即绝经后骨质流失率与作为习惯性饮食摄入标志的植物雌激素长期尿排泄量呈负相关。
对荷兰绝经后妇女进行的一项为期10年的随访研究（1979年至1989年）的二次分析。
从最初的154名女性群体中，挑选出32名在研究的前5年桡骨骨质流失率≤0.5%的女性，以及35名每年骨质流失率≥2.5%的女性。
通过气相色谱 - 质谱法测定每年收集的尿液样本混合样中的异黄酮染料木黄酮、大豆苷元和雌马酚，以及木脂素肠内酯。桡骨皮质骨密度此前每年通过单光子吸收法进行测量。
两组之间异黄酮的排泄没有差异，尽管在多变量分析中，雌马酚排泄与绝经后5年的骨质流失率呈弱正相关。骨质流失率高的组中肠内酯排泄显著更高。在对年龄、绝经年限、体重指数以及钙、植物蛋白和膳食纤维摄入量进行调整后的多变量线性回归分析中，这种正相关仍然存在。
肠内酯排泄可能是谷物和豆类消费的一个指标；尚不清楚观察到的与骨质流失率的正相关是否为因果关系。我们的结果不支持低剂量、未补充植物雌激素的饮食摄入对绝经后皮质骨流失具有预防作用。然而，对于更高剂量植物雌激素的影响无法得出结论。
