Kawabata T, Ota M, Nishikawa K
Center for Information Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.
Nucleic Acids Res. 1999 Jan 1;27(1):355-7. doi: 10.1093/nar/27.1.355.
Currently the protein mutant database (PMD) contains over 81 000 mutants, including artificial as well as natural mutants of various proteins extracted from about 10 000 articles. We recently developed a powerful viewing and retrieving system (http://pmd.ddbj.nig.ac.jp), which is integrated with the sequence and tertiary structure databases. The system has the following features: (i) mutated sequences are displayed after being automatically generated from the information described in the entry together with the sequence data of wild-type proteins integrated. This is a convenient feature because it allows one to see the position of altered amino acids (shown in a different color) in the entire sequence of a wild-type protein; (ii) for those proteins whose 3D structures have been experimentally determined, a 3D structure is displayed to show mutation sites in a different color; (iii) a sequence homology search against PMD can be carried out with any query sequence; (iv) a summary of mutations of homologous sequences can be displayed, which shows all the mutations at a certain site of a protein, recorded throughout the PMD.
目前，蛋白质突变体数据库（PMD）包含超过81000个突变体，包括从约10000篇文章中提取的各种蛋白质的人工和天然突变体。我们最近开发了一个强大的查看和检索系统（http://pmd.ddbj.nig.ac.jp），该系统与序列和三级结构数据库集成。该系统具有以下特点：（i）根据条目中描述的信息自动生成突变序列，并与整合的野生型蛋白质序列数据一起显示。这是一个方便的功能，因为它允许人们在野生型蛋白质的整个序列中看到改变的氨基酸位置（以不同颜色显示）；（ii）对于那些三维结构已通过实验确定的蛋白质，显示三维结构以不同颜色显示突变位点；（iii）可以使用任何查询序列对PMD进行序列同源性搜索；（iv）可以显示同源序列突变的摘要，该摘要显示了整个PMD中记录的蛋白质某个位点的所有突变。
