Hammer K A, Carson C F, Riley T V
Department of Microbiology, The University of Western Australia, The Queen Elizabeth II Medical Centre, Nedlands.
J Antimicrob Chemother. 1998 Nov;42(5):591-5. doi: 10.1093/jac/42.5.591.
The in-vitro activity of a range of essential oils, including tea tree oil, against the yeast candida was examined. Of the 24 essential oils tested by the agar dilution method against Candida albicans ATCC 10231, three did not inhibit C. albicans at the highest concentration tested, which was 2.0% (v/v) oil. Sandalwood oil had the lowest MIC, inhibiting C. albicans at 0.06%. Melaleuca alternifolia (tea tree) oil was investigated for activity against 81 C. albicans isolates and 33 non-albicans Candida isolates. By the broth microdilution method, the minimum concentration of oil inhibiting 90% of isolates for both C. albicans and non-albicans Candida species was 0.25% (v/v). The minimum concentration of oil killing 90% of isolates was 0.25% for C. albicans and 0.5% for non-albicans Candida species. Fifty-seven Candida isolates were tested for sensitivity to tea tree oil by the agar dilution method; the minimum concentration of oil inhibiting 90% of isolates was 0.5%. Tests on three intra-vaginal tea tree oil products showed these products to have MICs and minimum fungicidal concentrations comparable to those of non-formulated tea tree oil, indicating that the tea tree oil contained in these products has retained its anticandidal activity. These data indicate that some essential oils are active against Candida spp., suggesting that they may be useful in the topical treatment of superficial candida infections.
研究了包括茶树油在内的一系列精油对酵母念珠菌的体外活性。通过琼脂稀释法对24种精油针对白色念珠菌ATCC 10231进行测试，其中三种在最高测试浓度即2.0%（v/v）精油时未抑制白色念珠菌。檀香油的最低抑菌浓度（MIC）最低，在0.06%时抑制白色念珠菌。对互叶白千层（茶树）油针对81株白色念珠菌分离株和33株非白色念珠菌分离株的活性进行了研究。通过肉汤微量稀释法，抑制90%白色念珠菌和非白色念珠菌分离株的精油最低浓度为0.25%（v/v）。杀死90%分离株的精油最低浓度，白色念珠菌为0.25%，非白色念珠菌为0.5%。通过琼脂稀释法对57株念珠菌分离株进行了对茶树油的敏感性测试；抑制90%分离株的精油最低浓度为0.5%。对三种阴道用茶树油产品进行的测试表明，这些产品的最低抑菌浓度和最低杀菌浓度与未配制的茶树油相当，表明这些产品中所含的茶树油保留了其抗念珠菌活性。这些数据表明，一些精油对念珠菌属有活性，提示它们可能在浅表念珠菌感染的局部治疗中有用。
