el-Mekkawy S, Meselhy M R, Nakamura N, Tezuka Y, Hattori M, Kakiuchi N, Shimotohno K, Kawahata T, Otake T
Research Institute for Traditional Sino-Japanese Medicines, Toyama Medical and Pharmaceutical University, Japan.
Phytochemistry. 1998 Nov;49(6):1651-7. doi: 10.1016/s0031-9422(98)00254-4.
A new highly oxygenated triterpene named ganoderic acid alpha has been isolated from a methanol extract of the fruiting bodies of Ganoderma lucidum together with twelve known compounds. The structures of the isolated compounds were determined by spectroscopic means including 2D-NMR. Ganoderiol F and ganodermanontriol were found to be active as anti-HIV-1 agents with an inhibitory concentration of 7.8 micrograms ml-1 for both, and ganoderic acid B, ganoderiol B, ganoderic acid C1, 3 beta-5 alpha-dihydroxy-6 beta-methoxyergosta-7,22-diene, ganoderic acid alpha, ganoderic acid H and ganoderiol A were moderately active inhibitors against HIV-1 PR with a 50% inhibitory concentration of 0.17-0.23 mM.
从灵芝子实体的甲醇提取物中分离出一种名为灵芝酸α的新型高氧化三萜，同时还分离出了12种已知化合物。通过包括二维核磁共振在内的光谱手段确定了分离出的化合物的结构。发现灵芝醇F和灵芝烯三醇作为抗HIV-1药物具有活性，两者的抑制浓度均为7.8微克/毫升，灵芝酸B、灵芝醇B、灵芝酸C1、3β-5α-二羟基-6β-甲氧基麦角甾-7,22-二烯、灵芝酸α、灵芝酸H和灵芝醇A是对HIV-1蛋白酶的中度活性抑制剂，50%抑制浓度为0.17 - 0.23毫摩尔。
