小鼠Ia抗原和人类DR抗原：氨基末端序列的同源性。
Murine Ia and human DR antigens: homology of amino-terminal sequences.
作者信息
Allison J P, Walker L E, Russell W A, Pellegrino M A, Ferrone S, Reisfeld R A, Frelinger J A, Silver J
出版信息
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3953-6. doi: 10.1073/pnas.75.8.3953.
Murine Ia and human DR antigens were isolated and purified by immunoprecipitation and sodium dodecyl sulfate/polyacrylamide gel electrophoresis with allo- and xenoantisera, respectively. The I-A subregion antigen consists of two chains, designated Aalpha and Abeta, with molecular weights of 35,000 and 26,000, respectively. The I-C subregion antigen likewise consists of two chains, designated Calpha and Cbeta, with molecular weights of 32,000 and 29,000, respectively. Under nonreducing conditions, the Cbeta chain migrates appreciably more rapidly on sodium dodecyl sulfate/polyacrylamide gels than the reduced Cbeta chain, reflecting the presence of an intrachain disulfide bond. The human DR antigen is also a two-chain unit and contains DRalpha and DRbeta components with molecular weights of 34,000 and 28,000, respectively. The DRbeta chain migrates more rapidly before reduction than afterward, like the murine Cbeta chain. The DRbeta and Cbeta chains are also strikingly homologous if a single amino acid shift is imposed on one of those chains. Thus, human DR antigens strongly resemble the murine I-C subregion antigens.
分别使用同种异体和异种抗血清，通过免疫沉淀和十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分离并纯化了小鼠Ia抗原和人类DR抗原。I-A亚区抗原由两条链组成，分别命名为Aα和Aβ，分子量分别为35,000和26,000。I-C亚区抗原同样由两条链组成，分别命名为Cα和Cβ，分子量分别为32,000和29,000。在非还原条件下，Cβ链在十二烷基硫酸钠/聚丙烯酰胺凝胶上的迁移速度明显比还原后的Cβ链快，这反映了链内二硫键的存在。人类DR抗原也是一个双链单位，包含分子量分别为34,000和28,000的DRα和DRβ成分。与小鼠Cβ链一样，DRβ链在还原前的迁移速度比还原后快。如果对其中一条链进行单个氨基酸移位，DRβ链和Cβ链也具有显著的同源性。因此，人类DR抗原与小鼠I-C亚区抗原非常相似。
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