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在用于检测支原体的聚合酶链反应（PCR）中与坏死梭杆菌发生意外交叉反应。

Unexpected cross-reaction with Fusobacterium necrophorum in a PCR for detection of mycoplasmas.

作者信息

Jensen J S, Bruun B, Gahrn-Hansen B

机构信息

Mycoplasma Laboratory, Neisseria Department, Statens Serum Institut, Copenhagen, Denmark.

出版信息

J Clin Microbiol. 1999 Mar;37(3):828-9. doi: 10.1128/JCM.37.3.828-829.1999.

DOI:10.1128/JCM.37.3.828-829.1999
PMID:9986867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC84573/
Abstract

Cross-reactions with Fusobacterium necrophorum were found in a PCR designed for detection of a wide range of mycoplasma species. Twenty-five strains of Fusobacterium were examined; all 14 F. necrophorum strains reacted positively, whereas all 7 Fusobacterium nucleatum strains reacted negatively. Two strains that were not F. necrophorum yielded variable results.

摘要

在一项旨在检测多种支原体物种的聚合酶链反应（PCR）中发现了与坏死梭杆菌的交叉反应。对25株梭杆菌进行了检测；所有14株坏死梭杆菌菌株反应呈阳性，而所有7株具核梭杆菌菌株反应呈阴性。两株非坏死梭杆菌的菌株产生了不同的结果。

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Isolation of Mycoplasma genitalium strains from the male urethra.从男性尿道中分离生殖支原体菌株。
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