Zhou N N, Shortridge K F, Claas E C, Krauss S L, Webster R G
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
J Virol. 1999 Apr;73(4):3366-74. doi: 10.1128/JVI.73.4.3366-3374.1999.
The H5N1 avian influenza virus that killed 6 of 18 persons infected in Hong Kong in 1997 was transmitted directly from poultry to humans. Viral isolates from this outbreak may provide molecular clues to zoonotic transfer. Here we demonstrate that the H5N1 viruses circulating in poultry comprised two distinguishable phylogenetic lineages in all genes that were in very rapid evolution. When introduced into new hosts, influenza viruses usually undergo rapid alteration of their surface glycoproteins, especially in the hemagglutinin (HA). Surprisingly, these H5N1 isolates had a large proportion of amino acid changes in all gene products except in the HA. These viruses maybe reassortants each of whose HA gene is well adapted to domestic poultry while the rest of the genome arises from a different source. The consensus amino acid sequences of "internal" virion proteins reveal amino acids previously found in human strains. These human-specific amino acids may be important factors in zoonotic transmission.
1997年在香港感染的18人中导致6人死亡的H5N1禽流感病毒是直接从家禽传播给人类的。此次疫情中的病毒分离株可能为动物源性传播提供分子线索。在此，我们证明，在家禽中传播的H5N1病毒在所有基因中都包含两个可区分的系统发育谱系，且这些基因处于快速进化中。当流感病毒引入新宿主时，通常会经历其表面糖蛋白的快速改变，尤其是血凝素（HA）。令人惊讶的是，这些H5N1分离株除HA外，所有基因产物中都有很大比例的氨基酸变化。这些病毒可能是重配体，其每个HA基因都很好地适应了家禽，而基因组的其余部分则来自不同的来源。病毒“内部”粒子蛋白的共有氨基酸序列揭示了先前在人类毒株中发现的氨基酸。这些人类特异性氨基酸可能是动物源性传播中的重要因素。
