Bult H, Bonta I L
Agents Actions. 1976 Nov;6(6):712-20. doi: 10.1007/BF02026093.
The specificity of the isolated rabbit aorta for identification and assay of prostaglandin endoperoxides, formed during aggregation of platelet rich plasma (PRP) from rats, was investigated in the superfused organ cascade. The use of cumulative dose-response curves in this design is quicker than conventional step-wise dosing while delivering identical results. On the rabbit aorta, in contrast to the rat fundus strip, methysergide is too weak an antagonist to rule out responses due to 5-HT. After pretreatment with phenoxybenzamine, in the presence of methysergide, the rabbit aorta retains its sensitivity towards oxygenated fatty acids, while being virtually unreactive towards 5-HT. The prostaglandin endoperoxide PGH2 is more potent than prostaglandin E2 when assayed on the rabbit aorta, while on the rat fundus the situation is reversed. Combining the rabbit aorta and rat fundus strip with pre-filtration on Amberlite XAD-2 columns showed that during collagen induced aggregation rat PRP generated both a labile RCS-most probably a prostaglandin endoperoxide- and a lipophylic prostaglandin-like material, along with 5-HT.
在灌注器官级联反应中，研究了分离的兔主动脉对大鼠富含血小板血浆（PRP）聚集过程中形成的前列腺素内过氧化物进行鉴定和测定的特异性。在该设计中使用累积剂量 - 反应曲线比传统的逐步给药更快，同时能得出相同的结果。与大鼠胃底条不同，在兔主动脉上，麦角新碱作为拮抗剂作用太弱，无法排除5 - HT引起的反应。在用酚苄明预处理后，在麦角新碱存在的情况下，兔主动脉对氧化脂肪酸仍保持敏感性，而对5 - HT几乎无反应。在兔主动脉上进行测定时，前列腺素内过氧化物PGH2比前列腺素E2更有效，而在大鼠胃底条上情况则相反。将兔主动脉和大鼠胃底条与在Amberlite XAD - 2柱上进行预过滤相结合表明，在胶原诱导的聚集过程中，大鼠PRP产生了一种不稳定的RCS（很可能是一种前列腺素内过氧化物）和一种亲脂性前列腺素样物质，以及5 - HT。
