Dhar P, Ghosh S, Bhattacharyya D K
Department of Chemical Technology, University Colleges of Science and Technology, Calcutta University, West Bengal, India.
Lipids. 1999 Feb;34(2):109-14. doi: 10.1007/s11745-999-0343-2.
The present study examined the antioxidant activity of conjugated octadecatrienoic fatty acid (9 cis,11 trans,13 trans-18:3), alpha-eleostearic acid, of karela seed (Momordica charantia), fed to rats for 4 wk. The growth pattern of rats and the effect on plasma cholesterol and high density lipoprotein (HDL) cholesterol and peroxidation of plasma lipid, lipoprotein, eryhrocyte membrane, and liver lipid were measured. Rats were raised on diets containing sunflower oil mixed with three different levels of conjugated trienoic fatty acid (9c,11t,3t-18:3) 0.5, 2, and 10% by weight; the control group was raised with sunflower oil as dietary oil as the source of linoleic acid (9c,12c-18:2). The growth pattern of the three experimental groups of rats showed no significant difference compared to the control group of rats, but the group with 10% 9c,11t,13t-18:3 had slightly higher body weight than the control group of rats. Concentrations of total cholesterol, HDL-cholesterol, and non-HDL-cholesterol in plasma were similar in all four groups. Plasma lipid peroxidation was significantly lower in the case of 0.5% 9c,11t,13t-18:3 group than the control group and the 2 and 10% 9c,11t,13t-18:3 dietary groups as well. Lipoprotein oxidation susceptibility test with 0.5, 2, and 10% 9c,11t,13t-18:3 dietary groups was significantly less susceptible to lipoprotein peroxidation when compared with sunflower oil dietary group, and the dietary group with 0.5% 9c,11t,13t-18:3 showed least susceptibility. There was significant lowering in erythrocyte ghost membrane lipid peroxidation in the 0.5, 2, and 10% 9c,11t,13t-18:3 dietary groups compared to the sunflower oil groups. Nonenzymatic liver tissue lipid peroxidation was significantly lower in the group of rats raised on 0.5% 9c,11t,13t-18:3, but the groups on 2 and 10% 9c,11t,13t-18:3 acid did not show any significant difference compared with the control group of rats.
本研究检测了喂食大鼠4周的苦瓜籽（苦瓜）中共轭十八碳三烯酸（9顺式，11反式，13反式-18:3）即α-桐酸的抗氧化活性。测定了大鼠的生长模式以及其对血浆胆固醇、高密度脂蛋白（HDL）胆固醇的影响，还有血浆脂质、脂蛋白、红细胞膜和肝脏脂质的过氧化情况。大鼠分别饲养在含有与三种不同水平（按重量计0.5%、2%和10%）共轭三烯酸（9顺式，11反式，13反式-18:3）混合的向日葵油的日粮中；对照组则以向日葵油作为日粮油作为亚油酸（9顺式，12顺式-18:2）的来源进行饲养。三组实验大鼠的生长模式与对照组大鼠相比无显著差异，但10% 9顺式，11反式，13反式-18:3组的体重略高于对照组大鼠。所有四组大鼠血浆中的总胆固醇、HDL胆固醇和非HDL胆固醇浓度相似。0.5% 9顺式，11反式，13反式-18:3组的血浆脂质过氧化显著低于对照组以及2%和10% 9顺式，11反式，13反式-18:3日粮组。0.5%、2%和10% 9顺式，11反式，13反式-18:3日粮组的脂蛋白氧化敏感性试验表明，与向日葵油日粮组相比，其对脂蛋白过氧化的敏感性显著降低，且0.5% 9顺式，11反式，13反式-18:3日粮组的敏感性最低。与向日葵油组相比，0.5%、2%和10% 9顺式，11反式，13反式-18:3日粮组的红细胞血影膜脂质过氧化显著降低。0.5% 9顺式，11反式，13反式-18:3饲养的大鼠组的非酶促肝脏组织脂质过氧化显著降低，但2%和10% 9顺式，11反式，13反式-18:3酸组与对照组大鼠相比未显示出任何显著差异。
