用于鉴定肠杆菌科细菌的pathotec快速I-D系统的评估
Evaluation of the pathotec Rapid I-D system for identification of Enterobacteriaceae.
作者信息
Smith P B, Rhoden D L, Tomfohrde K M
出版信息
J Clin Microbiol. 1975 Apr;1(4):359-62. doi: 10.1128/jcm.1.4.359-362.1975.
Abstract
The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials. The PathoTec system exhibited 94.3% accuracy in identifying these cultures in a double-blind study with conventional media and procedures as the alternate system. Two newly developed test strips, for 0-nitrophenyl-beta-D-galactopyranoside and ornithine decarboxylase, were found to be highly reliable.
摘要
使用471份培养物对用于鉴定肠杆菌科细菌的PathoTec快速鉴定系统进行了评估。在4910次单独的测试比较中，95.5%的结果一致，只有两种测试条（七叶苷水解和尿素酶产生测试条）的结果在不到94%的试验中与传统测试结果一致。在一项双盲研究中，以传统培养基和程序作为对照系统，PathoTec系统在鉴定这些培养物时的准确率为94.3%。发现两种新开发的测试条（对硝基苯-β-D-吡喃半乳糖苷和鸟氨酸脱羧酶测试条）非常可靠。
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