Qadri S M, Johnson S, Smith J C, Zubairi S, Gillum R L
J Clin Microbiol. 1981 Mar;13(3):459-62. doi: 10.1128/jcm.13.3.459-462.1981.
The ability of several anaerobic bacteria to hydrolyze esculin to esculetin is used by clinical microbiologists and taxonomists in the differentiation and identification of both gram-positive and gram-negative microorganisms. Conventional methods used for determining esculin hydrolysis by anaerobic bacteria require 24 to 48 h for completion. In this paper we evaluate two procedures which yield rapid results. A total of 738 anaerobic bacteria were used in this study. A total of 99% of the esculin-hydrolyzing anaerobic bacteria gave positive results with the spot test in 1 h, whereas the other test method, the PathoTec strip test (General Diagnostics, Morris Plains, N.J.), required 4 h for 96% of the strains tested to yield positive reactions. Both tests showed a 100% specificity when compared with the standard broth test and are easy to perform, accurate, and economical. The spot test is superior to the PathoTec strip test in yielding results more rapidly.
临床微生物学家和分类学家利用几种厌氧菌将七叶苷水解为七叶亭的能力来鉴别革兰氏阳性和革兰氏阴性微生物。用于测定厌氧菌七叶苷水解的传统方法需要24至48小时才能完成。在本文中，我们评估了两种能快速得出结果的方法。本研究共使用了738株厌氧菌。共有99%的能水解七叶苷的厌氧菌在斑点试验中1小时内呈阳性结果，而另一种试验方法，即PathoTec试纸条试验（通用诊断公司，新泽西州莫里斯平），96%的受试菌株需要4小时才能产生阳性反应。与标准肉汤试验相比，这两种试验的特异性均为100%，且操作简便、准确、经济。斑点试验在更快得出结果方面优于PathoTec试纸条试验。
